Cell culture is the process of growing and maintaining living cells in vitro.

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What kind of detergent should I use in cell culture laboratory for cleaning purposes?

I work on establish a cell culture laboratory in the company where I work. All equipment are new and I want to clean lab before I start to work with cells. Is there any special detergent for cleaning ...
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why selecting cells after gene editing with CRISPR Cas9?

I am new to the CRISPR Cas9 genome editing system and I have the above basic question. Another way to think about this: what would happen if after cells are transfected, the cultures are left to grow ...
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Do bacteria grow on pure dry glucose?

I've accidentally touched pure glucose with my bare hands (fingers to be specific), which was intended for cell-culture. I'm worried that bacteria from my skin may start to grow on the glucose and ...
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Respiration of an animal cell media

I want to do respiration process of an animal cell media in laboratory environment. I don't entirely sure how to supply it with Oxygen to the process because it is a gas, but not a liquid.
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Can you replace glucose with glycerol in cell media?

In order to feed an animal cell in process called Respiration, can I replace Glucose with Glycerol? The Equation bellow: Glycerol + Oxygen -> Water + Carbon Oxide
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What a cell needs to survive? [closed]

I'm looking specifically at animal cells. I'm not sure if it needs only Glucose and Oxygen for Aerobic respiration.
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What does confluency mean?

Since as long as I have been doing cell culture, the word confluency is used to describe the % growth of cells or area covered by them. However, no dictionary that I have found uses this word. I was ...
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Confluency or confluence, which term is correct to describe the % area covered by cells?

I noticed that both of them are used in many scientific papers. Are these two terms, or can they be used interchangeably?
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how to make plasma cells adhere to the bottom of a microplate?

I am isolating single plasma cells by FACS sorting into 384-well plates, with the intent to assay the supernatant and clone H/L chains from positive wells. The efficiency of the PCR is however low, ...
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226 views

Formula for number of divisions required to form x number of cells (Mitosis and Meiosis)

In case of Mitotic Division, we say that if the total number of cells formed by mitotic divisions are $x$, then counting from the first cell onwards, we get $x-1$ divisions ($N$) $N = x-1$ This is ...
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How can I keep HEK cells alive while expressing NMDA receptors?

I am trying to express functional NMDA receptors in HEK293 line cells for single channel recording experiments. The HEK cells are maintained in the standard way (Thomas & Smart 2005) and ...
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How to induce a steady increase in cyctoplasmic [Ca2+] in HEK293T cells? And is there any simple marker/method to confirm it?

We are trying to find out whether increase or decrease of cytoplasmic calcium concentration affects the interaction of two proteins, using co-IP in HEK293T cells as a readout. In different forums and ...
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Is there a karyotype database for human cell lines?

I'm looking for the karyotype of a specific cell line. Some karyotype are well known, such as HeLa or some carcinomas, but some are very hard to find, such as LG2 (B cells). Is there a database ...
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Soft Agar Substitute

I've got petri dishes with a base agar layer ready for E. coli culturing. However, I do not have soft agar in which to dilute the E. coli before pouring it on to the plate. Any suggestions on what ...
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Alternative to Sterilizing Under Pressure

I want to grow mycelium in agar, and all the resources I have found say to sterilize the agar under 15 psi, but I do not have the necessary equipment to sterilize the agar under pressure. Is there an ...
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32 views

What are mouse LS cells?

I have seen the term "mouse LS cell" used in several papers now, but I haven't found an explanation for the origin of these cells (for example, this paper). What are mouse LS cells? What is their ...
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Why lactate inhibits growth (or enhances death rate)?

Extracellular lactate tends to inhibit cellular growth or enhance cell death. This happens in the vicinity of tumors and in cell cultures. See for example this reference: Ozturk, Sadettin S., Mark ...
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Does a specific blood group enhance the Plasmodium growth?

I am maintaining Plasmodium falciparum cultures for past 6 months. For the blood culture, usually we lab members take turns and donate blood for the culture. I observed that the parasite's normal ...
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Model for Oxygen diffusion into tissue? [closed]

Currently I have an idea and want to research it's feasibility. I want to know how oxygen or any other particle could diffuse into the body through small holes in the skin. is it possible to ...
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135 views

Two different sized colonies from the same species of bacteria. What does it mean? [closed]

I have got two different sized colonies in a plate of Salmonella Paratyphi, identified by biochemical methods. What does two different sized colonies mean? What kind of question can I investigate in ...
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Do you know of any scratch assay video databases?

It would be of great help for a statistical work we have been asked to carry out at our University. Thank you very much in advance.
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How sorbitol treatment selects ring stage of plasmodium falciparum

I would like to know the basic chemistry behind ring stage selection by sorbitol. Sorbitol is used for Synchronising the plasmodium culture in ring stage. How the chemical retains or selects ring ...
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What is the effect of exendin on beta-cells?

Do you know if exendin, an analog of GLP-1 (glucagon like peptide-1), can be toxic for beta-cells? For example, what is the effect on INS1 or Min6 cells at a certain concentration or after 90 mins of ...
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Gender of BEAS-2B cell culture

I recognize some interesting patterns when analysing DNA methylation of BEAS-2B cells. Does anybody know, if the cell culture is female or male? I already asked the customer support of Sigma Aldrich ...
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241 views

How long can a human neuron live outside the body in a controlled environment?

Have there been any experiments that have kept neurons alive (stationary), without preserving methods such as freezing? If yes, then how long were the cells kept alive for?
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UACC-462 cell culture

UACC-462 is a pancreatic cell line established in the University of Arizona Cancer Center (UACC). The cells are available from ATCC and there is the instruction for culturing UACC-462. M-41 medium. ...
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Is it possible to grow E. coli K12 with algal amino acids as the sole carbon source?

Instead of glucose or glycerol, is it possible for E coli to grow on an amino acid mixture as the sole carbon source and, if so, what should the concentration be?
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How to validate the use of an anti-virus monoclonal antibody in IHC by spiking a fresh organ with infected cultured cells?

I have assessed the specificity of a particular monoclonal antibody against a virus by immunofluorescence. I'd like to further validate it by testing it by IHC. Therefore I'd like to infect cells in ...
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yeast colonies pulsing out soft fringes against the light

I have no idea about this part of the following text "pulsing out soft fringes against the light". Can anybody explain about it and provide some images, please? Does it mean that these colonies ...
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Which human cell lines do not express the GLP-1 receptor?

I need a human cell line that does not express the GLP-1 (glucagon like peptide-1) receptor. I'm working with HeLa cells, do those express the GLP-1 receptor? Which other cell lines exist that don't ...
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147 views

Staining cells for FACS at 4 degrees or ambient temperature

I'm sorting a 293 derived line. One thing that is worrisome for me is cell viability after the sort. Usually I have been staining and washing at room temperature (on the benchtop) on a nutator. I have ...
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Jurkat single cell clonal expansion

I'm hoping to do a clonal expansion of Jurkat, expressing my construct which is currently transduced virally. From the FACS data I currently have roughly 30-40% expression in my culture but I need it ...
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378 views

Why are cell lines frozen in vapor phase?

For most of the cell lines I've come across, ATCC recommends storing them in the vapor phase of liquid nitrogen. I'm taking that to mean any place above the level of liquid in the nitrogen storage ...
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Testing CO₂ for cell culture

I'm am refurbishing some old CO2 incubators. I would like to test the precision of the CO2 sensor. There is a sample port on this incubator. What sorts of methods are commonly used to measure CO2 in ...
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What is the standard protocol for creating permanent stocks from freeze-dried bacteria?

I've received freeze-dried bacteria that I intend to use for a study I'm doing on slime mold; however, I've never dealt with freeze-dried, only frozen, bacterial cells. After reconstituting these ...
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Why do epithelial cells arrest in response to serum?

Primary epithelial cells, for example human mammary epithelium, fail to proliferate (arrest) in serum-containing medium. Therefore, a common growth medium for epithelium contains pituitary extract ...
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Cell line immortalisation biosafety

Cell lines are often immortalised by artificial expression of proteins which specifically cause the knockout of important cancer suppression genes or the activation of proto-oncogenes. Cellular ...
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How to grow fibroblast colonies from single cells?

I need to establish a cell line starting from single cells seeded on a 96 wells plate by a FACS sorter. The cells I am using are human fibroblasts RPE-1 cultured in F12 medium supplemented with 10% ...
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Trypsin and cell culture

I am doing an experiment where I have treat the cells with a drug and calculate their counts. I would like to know if is bad to trypsinize the cells in consecutive days i.e. twice within 48 hours. How ...
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Why do some protocols require prewarming a liquid medium before inoculating?

For example, in this protocol for E. coli competent cell preparation, it says: Plate 10 uL E. coli BL21(DE3) cells on a LB-agar plate; incubate overnight (12 hours). Prepare 500 mL SOB medium ...
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Do I need to serially dilute E. coli cultures for optical density measures?

I am expected to track cell growth by measuring the incubating culture's cell concentration every 30 minutes or so. So my questions are: Why do I need to do serial dilution (assuming that I do)? Why ...
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Are stem cell lines cryogenically preserved or commercially available like HeLa cells are?

There are HeLa cells, which were taken from sick cancer patient and now is growing worldwide in different laboratories for experiments. HeLa cells can be kept because they are immortal and so they are ...
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Can the Staph streak method be used for culturing Neisseria on blood agar?

What I learned in class: Normally chocolate agar is needed to grow Haemophilus and Neisseria. The Staph streak technique can be used for culturing Haemophilus on blood agar by allowing the Staph to ...
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Are HeLa cells edible?

I'm curious if HeLa cells are intrinsically poisonous or dangerous to ingest. My understanding is that some of the contamination in HeLa cells such as HPVs are not readily expressed. I have no plans ...
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Is there any alternative to cryopreserve cell lines without -80 freezer?

My university's -80 freezer just broke down and the repair will take 4 months according to the vendor. Is there any other way to preserve cells? Maintaining lot of flasks until the freezer is repaired ...
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291 views

Cationic lipid mediated transfection optimization for 150mm dishes

I need to optimize a transfection protocol to transiently express a plasmid encoding a chimera of eyfp attached to the c term of a Golgi apparatus signaling molecule) in hela cells and hepg2 cells ...
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419 views

Are cell lines potentially dangerous?

More specifically, if a human subject was exposed to, say, a human cancerous cell line (via intravenous injection or through an open wound, for example), is it possible that they would develop any ...
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128 views

What type of flask should I use to culture NTERA2 embryonic cancer stem cells?

I'm just starting my MSc research and I am in the process of making a list of equipments/consumables to order. Is there a specific flask in which I can culture NTERA2 (NTERA2/D1) cell line?
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What microscope/magnification would I need to observe P. Acnes bacteria?

I am currently attempting to grow a culture of P. Acnes bacteria. Right now, my only hope in identifying colonies of the bacteria in the culture is to use a black light to find colonies that glow ...
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What temperature should mammalian B-Cells be stored at outside of the incubator?

I'm working with murine B-cells. The general protocol is to keep cells on ice to keep them from dying but I've noticed that it makes these cells aggregate and precipitate out. I've heard suggestions ...