Cell culture is the process of growing and maintaining living cells in vitro.
5
votes
0answers
44 views
What temperature should mammalian B-Cells be stored at outside of the incubator?
I'm working with murine B-cells. The general protocol is to keep cells on ice to keep them from dying but I've noticed that it makes these cells aggregate and precipitate out. I've heard suggestions ...
5
votes
0answers
30 views
Providing small molecules to cells on a filter plate
Lets imagine that I have mammalian cells that I've immobilized on a filter. Now I want to keep providing small molecules to these immobilized cells without resolubilizing the cells.
The caveat is ...
3
votes
0answers
26 views
Can a cell culture insert plate be used with a vacuum manifold?
There exists 96 well plate setups for use with vacuum manifolds. Is it possible to use 6/12/24 plate inserts with the same setup?
2
votes
0answers
33 views
Where to put the gene after eukaryotic promoter for best expression levels?
As far as I know there is an optimum distance between a promoter and the gene for the best expression levels. What is that distance for common promoters like CMV, SV40? If you have a first hand ...
2
votes
0answers
39 views
Resuspending Cells from a filter plate
My experiment involves pulling down cells on to a filter plate for a variety of assays. However, I want to ensure that my cells are alive so I am trying to resuspend them so that I can do an accurate ...
1
vote
0answers
21 views
What mammalian cell lines are easiest to grow and transfect?
I'm working with HEK293 cells, which are very easy to transfect. Does anyone have other suggestions for cell lines with very high transfection efficiency? Ideally the chromosomes would also be diploid ...
1
vote
0answers
131 views
Appropriate methods for triteration?
What is the appropriate method to pipetting up and down mammalians cells to sufficiently break up clumps. This should also keep in mind the issues from Pipetting damage on cells. Visually one can see ...
