Cell culture is the process of growing and maintaining living cells in vitro.

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Is there a karyotype database for human cell lines?

I'm looking for the karyotype of a specific cell line. Some karyotype are well known, such as HeLa or some carcinomas, but some are very hard to find, such as LG2 (B cells). Is there a database ...
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242 views

Resuspending Cells from a filter plate

My experiment involves pulling down cells on to a filter plate for a variety of assays. However, I want to ensure that my cells are alive so I am trying to resuspend them so that I can do an accurate ...
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how to make plasma cells adhere to the bottom of a microplate?

I am isolating single plasma cells by FACS sorting into 384-well plates, with the intent to assay the supernatant and clone H/L chains from positive wells. The efficiency of the PCR is however low, ...
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Why lactate inhibits growth (or enhances death rate)?

Extracellular lactate tends to inhibit cellular growth or enhance cell death. This happens in the vicinity of tumors and in cell cultures. See for example this reference: Ozturk, Sadettin S., Mark R....
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What is the standard protocol for creating permanent stocks from freeze-dried bacteria?

I've received freeze-dried bacteria that I intend to use for a study I'm doing on slime mold; however, I've never dealt with freeze-dried, only frozen, bacterial cells. After reconstituting these ...
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Parafilm vs Foil for Retaining Plate Moisture while Allowing Gas Exchange

We often plate Streptomyces aureofaciens and grow them in a 30°C room. A post-doc I work with has always loosely wrapped the agar plates in aluminum foil to retain moisture and prevent cracking of the ...
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Effective Mycoplasma Elimination from Primary Human Cultures

Obviously the best way to avoid mycoplasma contamination is to avoid it in the first place. In our case, however, it is not possible to avoid. We are culturing viruses and tissues out of human nasal ...
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How does air-liquid interface (ALI) culture work?

For studying diseases, such as chronic obstructive pulmonary disease (COPD), cells are often grown in air liquid interface. I understand that the common way of establishing this these days is to grow ...
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Effect of flask position in shaking incubator affects growth kinetics

During some cultivation experiments for my bachelor thesis I noticed that in liquid culture my bacteria (E. coli) grew differently, according to the position in the shaking incubator. Because the ...
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Creating Sperm via cell culture adaptation

I am wondering if it is theoretically possible to create "artificial sperm" via cell culture adaptation. That is at very high level, can you replace the genes of one sperm cell with those of another ...
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Are HEK293 cells a positive control for wild type p53 expression?

I have searched in the web and found that these two antibodies from abcam use hek293 as positive control. * ab154036 * ab1101 But I am using another antibody: * AM11182PU-S (OriGene Technologies Inc.)...
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why selecting cells after gene editing with CRISPR Cas9?

I am new to the CRISPR Cas9 genome editing system and I have the above basic question. Another way to think about this: what would happen if after cells are transfected, the cultures are left to grow ...
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Alternative to Sterilizing Under Pressure

I want to grow mycelium in agar, and all the resources I have found say to sterilize the agar under 15 psi, but I do not have the necessary equipment to sterilize the agar under pressure. Is there an ...
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How sorbitol treatment selects ring stage of plasmodium falciparum

I would like to know the basic chemistry behind ring stage selection by sorbitol. Sorbitol is used for Synchronising the plasmodium culture in ring stage. How the chemical retains or selects ring ...
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38 views

UACC-462 cell culture

UACC-462 is a pancreatic cell line established in the University of Arizona Cancer Center (UACC). The cells are available from ATCC and there is the instruction for culturing UACC-462. M-41 medium. ...
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Do you know of any scratch assay video databases?

It would be of great help for a statistical work we have been asked to carry out at our University. Thank you very much in advance.
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yeast colonies pulsing out soft fringes against the light

I have no idea about this part of the following text "pulsing out soft fringes against the light". Can anybody explain about it and provide some images, please? Does it mean that these colonies ...
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138 views

Jurkat single cell clonal expansion

I'm hoping to do a clonal expansion of Jurkat, expressing my construct which is currently transduced virally. From the FACS data I currently have roughly 30-40% expression in my culture but I need it ...