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optimization of density gradient centrifugation

I'm trying to isolate a ~320kD protein (in oligomeric form) using density gradient ultra centrifugation technique. Can any one tell me that what gradient (sucrose or glycerol or trehalose or etc) I ...
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What is the sedimentation coefficient of Mycoplasma?

I have attempted to find the sedimentation coefficient of a number of bacterial species, namely E. coli and a number of Mycoplasma species, but they seemed to be rather elusive in the literature. If ...
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Estimating RPM to RCF in Methods from Older Papers

I'm attempting to replicate a cell biology method from a 1958 Laboratory Investigation paper. The protocol is for the isolation of an extracellular matrix protein, and a key step is a centrifugation ...
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3k views

What's the physical meaning of Svedberg unit?

Sedimentation coefficients, using a centrifuge, are expressed using Svedberg unit (symbol S, sometimes Sv). Wikipedia states that $S = 10^{-13}$ sec but I also saw in a book that actually $S = 10^{-13}...
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Changing time and rpm of centifuge

Inspired by this question, I want to ask a question about centrifugation. Suppose a protocol says : 10 min at 2500 rpm . Can we instead centifuge for 5 min at 5000 rpm or 20 min at 1250 rpm ?