0
votes
0answers
46 views

How can I get higher concentrations of PCR products

I am trying to multi-fragment Gibson Assemblies and my PCR product concentrations are not high enough to yield plasmids every time. I am using a very low amount of elution buffer to maximise ...
3
votes
0answers
73 views

How does LCR compare to Assembly PCR

The question pretty much explains itself. How do the two methods compare? I've always used Assembly PCR but the method is prone to mistakes and I'm curious how it compares to Ligase Chain Reaction ...
11
votes
1answer
247 views

Deletion errors with Phusion Polymerase?

I was wondering if anyone has ever seen large deletions (~40-50 bp) occur when using phusion polymerase to clone a gene. I have recently tried using Circular Polymerase Extension cloning to clone a ...