Questions tagged [cloning]
The process in nature or in the lab by which a new organism is created that is genetically identical to its predecessor.
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Does a man contain all the genes needed to make a woman?
This question is brought on by a Sci Fi novel I am thinking about writing. The plot device involves a colonist in charge of building a population on a new planet who loses his supply of embryos and so ...
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Can a human be made with ovum but without sperm?
This article says that scientists were successful in making a mouse using only a sperm and no egg (ovum). The article also states that this procedure could be applicable to humans.
However, I want to ...
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How to DIY preserve pet DNA today so that it can be used in 20 years
I know that there are companies that offer a kit+storage of your pet DNA for around $2k.
My question is if there is any other option for doing this yourself with a thought in mind that in 20+ years ...
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Why are female clones more often produced
As a student of biology when ever I come by artificial cloning, I always find examples of females being cloned - Dolly the sheep, CopyCat, Daisy, etc. The only male I could see was Fibro mouse and a ...
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In Sanger sequencing, why do we resort to cloning? Why doesn't PCR suffice?
I understand that in Sanger sequencing we can clone our fragments with the help of e.g. bacteria to make multiple copies of our fragments for further analysis.
I also understand cloning can be a ...
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Why does one combine PCR and cloning as ways for amplification of sequences?
Why does one combine PCR and cloning as ways for amplification of sequences? Don't they produce the same result? I was reading the paper https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1864885/ and got ...
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Is SCNT possible with only the nucleus of a somatic cell (rather than a whole somatic cell)?
The following figure from Life: The Science of Biology (11th edition) explains how SCNT (somatic cell nuclear transfer) was used in the cloning of Dolly:
(The original paper about cloning Dolly - ...
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How to specify a promoter in de novo gene synthesis service?
I am trying to use a de novo gene synthesis service (Genscript) and one part confuses me:
Where do I pick the choice of promoter? Or is the promoter choice automatic based on my plasmid choice?
e.g....