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4
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1answer
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Why am I getting low transformation efficiency with DB3.1 E.coli cells?
I am making competent cells using DB3.1 E. coli cells. Even after following the exact protocol (Inoue method for ultracompetent cells) given in 'Sambrook and Russel', I am not getting transformation ...
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0answers
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Cell growth conditions for preparing electrocompetent cells
Usually the protocol for preparing electrocompetent E. coli cells calls for growing the cells at 37deg and 225rpms until they reach OD of 0.3. I was wondering, is there any reason they should grow at ...
