DIY biology is a movement of hobby biologists practicing outside of mainstream labs. This means that budget, availability of technology, and possibly background knowledge may be minimal.

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5
votes
2answers
364 views

Can PCR tubes be made of different materials than plastic?

Does it matter if I replace the PCR tube (usually made of plastic) with another material like aluminum, glass, or something similar?
0
votes
1answer
68 views

Real Time PCR Test Chemistry

I am working on building a real time PCR machine. Is there any chemistry set that I can purchase online to: Identify if PCR amplification was successfully done. Test fluorescence dyes that could ...
0
votes
0answers
19 views

Is it possible to inject pigments into a leaf?

Is it possible to introduce exogenic pigments into the leaves of a plant? And, if so, how could you do that? Goals: the plant should not die the pigment should be visible looking at the plant (...
11
votes
1answer
5k views

How to obtain bacteria samples at home?

As the original question went from hold to closed, I thought I would write up a more appropriate question. How should one go about getting bacterial samples to look at under a microscope at home? ...
2
votes
2answers
82 views

How to obtain a tardigrade?

The tardigrade is often mentioned as one of the most "alien" lifeforms on Earth. This video speaks for itself: (from this article) I have long wanted to see a tardigrade with my own eyes. I have a ...
2
votes
3answers
560 views

How can I create a microcapillary for manipulation of single cells?

i'm working as a diy bio. I'm finding a way to create a micro glass capillary for picking up single cells. I see this video on youtube and would like to know what is the minimum I/O diameter if I pull ...
-5
votes
1answer
445 views

Can genetic engineering be performed without a laboratory setting? [closed]

I'm becoming more interested in genetic engineering as I learn more about it, and it's becoming more and more apparent that there's very few resources on genetic engineering at home. A similar ...
2
votes
1answer
447 views

DIY extraction and long term storage of human DNA sample?

What is the recommended DIY way of extracting and long term storing of human DNA samples to store at home and send for sequencing in years time? Considerations: Sample material: cheek swab, hair, ...
4
votes
2answers
137 views

Detecting food fraud

There's undoubtedly more than one way to do this, but if a DIY biologist were to attempt to detect food fraud (e.g. as done by students from Stanford University and Trinity School, Manhattan with ...
2
votes
2answers
885 views

Beginning with centrifuge experiment

This is the first time I do a centrifuge experiment with my own centrifuge machine, it's just simple made by a rotor that can rotate the tube at high speed. As I know a centrifuge machine can be using ...
1
vote
2answers
199 views

Finding a easy and cheap method for dyeing dNTP

I want to measure OD to know the concentration of dNTP. Any idea for dyeing dNTP at cheapest price and easiest way ?
1
vote
1answer
175 views

Preparation of normal DNA polymerase

i don't need Taq polymerase. Just need normal DNA polymerase. So the main idea is preparation polymerase directly from Lactobacillus without using cloned vector. My idea is electrophoresis using ...
0
votes
1answer
75 views

Some question about materials for PCR

I'm planning to order oligo, polymerase, nucleotide, buffer for my first diy PCR experiment (now I have nothing), so I have some questions. How and how long I can storage them? I have only a fridge ...
0
votes
3answers
157 views

Pump to separate protein/DNA instead of electrophoresis?

I know that DNA molecules or proteins can be separated in electrophoresis because the electrical charge is used to pull the DNA through the gel. So instead of using electrical, can I using physical ...
5
votes
3answers
150 views

Finding a template/oligo combination for my first PCR experiment

I'm an information technology engineer. I love biology so I research biological topics and have an interest in PCR. That's why I have decided to create a PCR machine. Everything is done now and I ...
4
votes
1answer
197 views

Effect of doubling volumes of PCR reagents

We ran PCR with a positive control and two lanes of sample. We used the same sample, but in one PCR mixture we used a total volume of 50μL (25μL Taq, 5μL forward&reverse primer each, 10&...