DIY biology is a movement of hobby biologists practicing outside of mainstream labs. This means that budget, availability of technology and possibly background knowledge may be minimal.
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Effect of doubling volumes of PCR reagents
We ran PCR with a positive control and two lanes of sample. We used the same sample, but in one PCR mixture we used a total volume of 50μL (25μL Taq, 5μL forward&reverse primer each, ...
