Deoxyribonucleic acid (DNA) is the carrier of genetic information, including for all known living organisms. The only known exceptions are RNA viruses.

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How is the protein removed from the DNA preparation? [on hold]

to determine how much DNA you have, you measure the absorption of the DNA.
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G>T transversion VS. T>G transversion?

So I'm reading about how mutations in DNA can be caused by oxidative damage. An example of a product of oxidative damage is given: 8-oxo-7-hydrodeoxyguanosine My textbook says that this product ...
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25 views

Why do the major and minor groove exist in DNA?

So I know that the major/minor groove arise from the offset pairing of the two strands in the helix. All my textbook tells me is that "pitch causes the off-set pairing of DNA and this gives rise to ...
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Physiological markers of some antibiotic resistance and DNA? [on hold]

I am studying antibiotic resistance through DNA on Physiology. I am particularly interested its effect on Physiology: heart rate blood pressure pacemakers of the heart mean electrical axis ...
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All cells in an embryo have the same heredity, yet they develop into different tissues and organs and organ systems. [closed]

To Remi.b All cells in an embryo have the same heredity, yet they develop into different tissues and organs and organ systems. What is the process by which this happens? THANKS :) (is it a proper ...
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81 views

Why deoxyribose for DNA and ribose for RNA?

Why is DNA made out of deoxyribose and RNA made of ribose? Why can't they both use ribose or deoxyribose? I think that the deoxyribose gives an advantage in storing genes, the job of DNA and ribose is ...
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60 views

Why does DNA have its name?

Why is DNA called deoxyribonucleic acid and not something else? I get the nucleic acid part (because that's what DNA is made of) but what about the deoxyribo- part, especially the ribo- part. Maybe ...
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30 views

DNA densities in different sources of the human body [closed]

I'm interested to know if there is any research in the density of DNA in different sources of the human body. By DNA density I mean the number of bonded between the nucleotides per mass or volume of ...
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43 views

What is the origin of DNA? [closed]

Deoxyribose nucleic acid underpins most life on this earth. It seems to me that the structure of DNA is already reasonably complicated and a certain degree of evolution may have already occured rather ...
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26 views

PCR cycle problem [closed]

IF I began a PCR cycle with 5 copies of a particular DNA section, and copied the section by PCR, for 6 cycles, how many copies of the DNA (include the originals) would I have by the end of these ...
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107 views

What makes DNA sticky-ends sticky?

When restriction enzymes jaggedly cut double stranded DNA it results in so called sticky ends. What is the substance that makes the DNA sticky?
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38 views

Low copy numbers of plasmids

I have a plasmid with the P15A origin which apparently has a low copy number (see here). This would explain why my purification yeilds for subsequent digestion are low (gel shows the plasmid after ...
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1answer
53 views

Are there any similarities in the DNA of a humans and animals? [closed]

As the title says, i've been wondering if there are any similarities between the DNA of humans and the DNA of animals? Like, maybe monkeys are more similiar in their DNA to humans, than dogs are (to ...
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515 views

Why does A pair with T and G with C?

DNA is made up of pairs on AT and GC base pairs. I know that A only pairs with T and G only with C. Does this apply just to humans, or are there animals where T will pair with G? Also, surely there ...
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1answer
52 views

Calculating number of amino acids in mRNA

Assuming there were 20 different amino acids and less than 40 types of different tRNAs found in this alien organism. How many amino acids would be found in the translational product of a ...
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1answer
25 views

Is the nuclear envelope present in G1 of interphase?

Is the nuclear envelope present in G1 of interphase of eukaryotic cell? If so how does a method like Calcium mediated transfection get DNA past the nuclear envelope?
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172 views

Importance of Double Helix DNA Structure

Gene expression involves transcribing only one strand of DNA molecule. So i was wondering what are some biological advantages of the double stranded DNA?
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125 views

Why is the threshold between human embryo and human fetus defined as 8 weeks after fertilization?

Why is the threshold between human embryo and human fetus defined as 8 weeks after fertilization? What's happening on the 8th week? Note: definition of embryo based on Wikipedia's current ...
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1answer
54 views

Has DNA communication/interaction at a distance been empirically demonstrated?

I recently had a discussion with a statistician in my school about memory. Among other things, he told me that it has been experimentally demonstrated that when one extracts DNA from any organism and ...
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Nucleosome wrapping direction

Does anyone know which direction the nucleosomes are wrapped? I wonder: Relative to the B-DNA double helix twist direction (right-hand) Relative to the neighbor nucleosomes. Do they alternate to ...
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1answer
27 views

Recovery from a more severe injury can be shorter than from a less severe one [closed]

I vaguely remember reading somewhere that in certain cases, recovery from a more severe injury can occur faster than from a less severe injury. This was motivated by claiming that the less severe ...
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1answer
63 views

Redundancy of the genetic code

One particular codon codes only for one amino acid, but an amino acid can be coded for by several different codons. Now according to the genetic code, the codon UUU ...
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2answers
62 views

What kind of technology is required for plant seed DNA printing? [closed]

I'm a programmer so I have little knowledge in the field of biology. However I have an interest in plants, GMOs and DNA (in terms of programming). So, I want to know what kind of technology is ...
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2answers
54 views

How does non-homologous end joining (NHEJ) work?

I was reading about non-homologous end joining (NHEJ) in my molecular biology of the gene textbook but the explanation provided in the text was rather vague to me, and I was not able to understand it ...
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How does high-fidelity of DNA replication depend on the formation of hydrogen bonds?

Replication has an error rate of less than 1 in 100 million. DNA polymerase forms H-bond with the H-bond acceptor atoms in the minor groove. <-- enhance fidelity here? Binding of the triphosphate ...
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Does DNA replication in 5' to 3' (leading strand) need RNA primase?

https://www.youtube.com/watch?v=27TxKoFU2Nw In the above video it shows that during DNA replication, the lagging strand require RNA primase to add 3' -OH group for further addition of nucleotides. ...
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Gibson assembly - primer design with A and T rich regions

I have question about Gibson assembly. I have done it several times and it always worked okay for us, but now I want to assemble together a fragment which has sequence like this: ...
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67 views

If DNA is the input, what is the output? [closed]

How does cells use the DNA inside them? I am looking on DNA as a strip of tape of Turing machine, but with machines its easy. I can read the tape and calculate the behavior of that machine. With cells ...
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48 views

Why do our epiphyseal plates close up in our late teens or early twenties?

What causes our epiphyseal plates close up in our late teens or early twenties? I realize that one's genetics plays the main role in this. I assume there is a gene that controls the epiphyseal plates ...
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Are the DNA codon mappings arbitrary?

I am a Biology novice so I apologize in advance if I state anything incorrectly.... My question is, are the DNA codon mappings arbitrary? I understand that the DNA code is made up of codons which ...
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34 views

programmatic fast search of 100-1000 short reads to a public server and obtain list of nearby genes

What are the options for programmatic fast searching of 100-1000 short reads to a public server and obtain list of nearby genes where the reads map? Input: ~100-1000 short reads Output: GFF list of ...
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1answer
44 views

How to identify genes?

You and me are different at DNA level. My eye color gene is different from yours. So, my DNA is different from yours. How can a scientist identify a certain gene in a chromosome (and its function) if ...
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1answer
68 views

5,6-dihydrouracil and 5,6-dihydroxyuracil - how they arise in DNA?

5,6-dihydrouracil can be formed from cytosine after exposure of DNA to ionizing radiation under anoxic conditions [Ref]. What are other ways by which 5,6-dihydrouracil is formed in DNA? What about ...
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1answer
56 views

What percentage of DNA do people share?

If you picked two people in the world at random, sequenced each of their entire genome, and compared their DNA, what would the expected percentage match be?
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49 views

How is the rate of transcription influenced by temperature?

How is the rate of transcription influenced by temperature? More precisely, I am looking for an article who quantitatively measured the rate of transcription of an "average gene" and show how this ...
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Transcription rate expressed in microarray per hour

This article gives measurement of transcription rate and the unit they're using is microarray per hour. For example, at 27°C the average expression of their genes is 236.1 microarray per hour (page ...
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where to find the relative frequency distribution of synonymous codons

Most amino acids can be encoded by more than one codon. For example, Serine can be encoded by any one of ...
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47 views

Chromatids in metaphase?

Please see the following picture: In my book, the author claims that these chromosomes are in metaphase (a metaphase stopped by cholchicin). I don't understand why they don't have two ...
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1answer
64 views

Has it been confirmed yet that the DNA found in dinosaurs was really DNA?

I hear different sources claiming different things, bbc said they did, wikipedia says it hasn't which is it?
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79 views

What causes skewed ladder bands on an agarose gel?

I frequently ran into an issue where the bands of my ladder become skewed. What causes this? Here's an example: 0.7% agarose gel, 100V, 60 minutes. Same problem occurs with 1%. I loaded 10 ul of a 20 ...
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Is there a biological mechanism for evolution encoded into our DNA?

Throughout high school, I remember learning about Darwin's theory of evolution as if it were near-fact. But something always seemed wrong about the ideas presented. Survival of the fittest Random ...
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1answer
31 views

How do nicks in the DNA strand affect the success of Long Range PCR?

Long Range PCR using NEB Master Mix - Hot Start Taq was working fine for me (amplicon sizes of ~10kb) but stopped working all of a sudden. Is it possible that many freeze-thaw cycles on the DNA ...
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DNA extraction from agarose gel

Because DNA is soluble in water, is it possible to extract PCR product by dissolving excised gel containing the DNA band of desired length in water, mashing the gel piece with a pestle, centrifuging ...
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1answer
34 views

What is the biding site code recognized by the parts of the spliceosome

Another question about another Youtube video. At 0:50, the splicing process begins to remove the non-coding section of the DNA (intron), so the different parts of the spliceosome attach to the borders ...
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1answer
48 views

What regulates the timing of the motion of molecular machines during DNA Replication?

This question is about this video I found on Youtube. I just want to know what is the mechanism which regulates the timing of motion of the parts of these molecular machines. I know that those big ...
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3answers
68 views

What is the purpose of DNA? [closed]

My very basic understanding is DNA gets transcribed into RNA by effectively replacing thymine with uracil, then RNA is translated to make proteins. Looking at it from an engineering perspective, it ...
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39 views

Mechanism of DNA gyrase inhibitor

Why DNA gyrase inhibitor, such as Nalidixic acid and Norfloxacin, do not stop gyrase from cutting DNA helix but only prevent them from decatenation replicating DNA?
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1answer
57 views

Can we attack viruses by attaching proteins and such to their shells?

Haven't looked into this to very long, but an initial search didn't return anything. HIV uses certain co-receptors to enter our cells. Could we flood the blood stream with specially designed ...
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374 views

Possible reasons for DNA getting stuck in well

I am in the process of Library preparation for Illumina MI-Seq using mtDNA. Using NEB Hotstart LongAmp Polymerase, I was able to obtain upto 10kb amplicons of mtDNA. However, when I switched to a ...