Deoxyribonucleic acid (DNA) is the carrier of genetic information, including for all known living organisms. The only known exceptions are RNA viruses.

learn more… | top users | synonyms

0
votes
1answer
16 views

Bioinformatics - DNA binding, sequence-specific protein data

I am a computer scientist and we are working on protein function prediction algorithms. Right now, we would like to examine protein-DNA binding properties computationaly. For this, we would make use ...
0
votes
1answer
21 views

Nature of Dengue Fever Genetic Material

Once the genetic material for Dengue Fever is inserted into the human DNA, would the Dengue Fever molecular material be in the form of its own isolated DNA fragment or will it be inserted directly ...
5
votes
3answers
118 views

Why does nature use a 4-level system (DNA) to encode information?

First, I am not a biologist, so this question might be naive: All of our information processing and storing is based on 2-level logic, bits with 0 and 1. Now, DNA stores the information in a 4-level ...
0
votes
0answers
17 views

Link together all alike DNA sequences [on hold]

Is it possible to take a DNA solution and link together every alike fragment of DNA in the solution?
1
vote
2answers
26 views

Is it possible to amplify every single piece of DNA through PCR?

Is there a way to perform non-specific PCR amplification for the purpose of amplifying every piece of DNA present?
0
votes
1answer
16 views

Extending a small fragment of DNA

Is there a way to extend a small fragment of DNA, say 150 bp, by making copies of itself and attaching each copy of that small fragment to the end of that 150 bp sequence? For example, I want a 1kbp+ ...
3
votes
3answers
115 views

Why does all life use the same macromolecules in their genetic code?

There is no biochemical constraint of any sort, so why doesn't some other code work? Why is it specifically RNA/DNA?
1
vote
2answers
33 views

Are genes on the 5' to 3' strand only?

I confused myself during studying, and wanted to confirm something. Since transcription via RNA polymerases only takes place in the 5'to 3' direction, that would mean that that 5' to 3' strand is the ...
0
votes
0answers
17 views

Relations of Watson & Crick work and Meselson & Stahl [closed]

It is said that Watson & Crick postulated the DNA model,does this make Meselson & Stahl (which found out that DNA is semi-conservative) contributed to enhance the postulate by Watson & ...
1
vote
0answers
22 views

There are 6 classifications of CFTR mutations. Is a causal relationship to the sweat test known?

Cystic fibrosis (CF) is caused by mutations in the gene for the protein cystic fibrosis transmembrane conductance regulator (CFTR). The CFTR mutations are classified in 6 classes. The sweat test is ...
3
votes
2answers
42 views

Organ donation compatibility based on DNA

As far as I know, multiple tests are made before organ transplant to determine matching. Would it be possible to do the matching based on the DNA of the patients, rather than the actual serum ...
0
votes
0answers
13 views

Diffuse DAPI staining after time in tissue

My student is using ProLong Gold with DAPI (premixed) on sheep uterine tissue. We have used this reagent for some time with great results. Now, when she finishes her immunofluorescence protocol, ...
3
votes
1answer
32 views

Are genes uniformly dispersed throughout the genome?

I think that telomeres and centromeres are regions with a very low gene content (= regions that contain few genes). To the exception of telomeres and centromeres, are genes uniformly distributed ...
2
votes
1answer
36 views

Why would mutation rates increase in a tumour?

This article describes a tumour: Swanton found that even the primary tumour was surprisingly varied. He found 128 mutations among the various samples, but only a third of these were common to all ...
2
votes
0answers
40 views

What happens when a genome is shorter than the other? [closed]

Say there were 2 creatures of the same species. Creature 1 has a longer genome than creature 2, it may be just a few base pairs, but what would happen when the genes were crossed to create creature 3 ...
0
votes
2answers
85 views

What is the DNA Sequence for an apple?

The title says it all. I'm just curious. I read that scientists mapped the genome for Malus Domestica, but I can't find a sequence anywhere.If this is a stupid question, I would appreciate if you tell ...
2
votes
1answer
123 views

Why are nitrogenous bases of DNA hydrophobic if they can hydrogen bond?

Why are nitrogenous bases of DNA hydrophobic if they can hydrogen bond? Is it that they are only relatively hydrophobic? This forum explains it but does not give an example of the structure.
3
votes
1answer
77 views

Is nuclear DNA immuno-privileged?

It is well known that if DNA occurs in the cytoplasm of eukaryotic cells, an immune response may be triggered through a myriad of DNA receptors and pathways as part of the immuno response. Yet, ...
1
vote
1answer
67 views

Is each generation getting older? [duplicate]

So we know that our sperm and egg cells get set aside relatively early so that they aren't going through unnecessary cell divisions and causing DNA damage or telomere shortening, but since each new ...
0
votes
0answers
29 views

Does bromophenol blue cause a shadow on agarose gels stained with SYBR Gold?

Bromophenol blue in loading dye is known to cast shadows/absorb emitted light signal from DNA on Ethidium bromide gels during imaging. But if SYBR Gold is instead used as the nucleic acid stain, does ...
0
votes
1answer
29 views

C Form DNA Base Pairs Per Turn

How does C-DNA have 9.33 base pairs per turn? The number of base pairs should be quantised. How can it be a decimal?
7
votes
1answer
43 views

Evolution of the Redundancy of the Genetic Code

In short Looking at the genetic code, it appears that most redundancy is on the third letter rather than on the first or the second letter of the codon. Why has it evolved this way? Longer version ...
10
votes
2answers
952 views

Linear and Circular DNA

So school has taught us that eukaryotes have DNA that is linear and inside of a membrane - called the nucleus. And that prokaryotes have circular DNA that is free floating inside of the cell. We ...
2
votes
2answers
91 views

Why do many DNA solutions contain additional compounds?

DNA solubility data in only water is scarce. A previous question asked for a quantification of DNA solubility in water. It seemed like it would be easily answerable, however isn't quite that simple ...
0
votes
1answer
24 views

Which pair of primers should be used to amplify the ORF in PCR? [closed]

So I want to choose the correct set of pair of primers to amplify the ORF of the gene that corresponds to amino acids in a protein. The start and stop codons are underlined. (I know that these need to ...
-2
votes
3answers
123 views

Immortality Gene? Really? [closed]

I read somewhere in the internet and wondered if human genes have deactivated immortality genes locked somewhere in the DNA strand. Is this statement true? What does it mean for human lifespan?
5
votes
1answer
387 views

What does it mean to “map the human genome”

I know some elementary chemistry and biology. I also think I know what a gene is (it's a sequence of DNA which encodes a particular protein). I also know that on a chromosome there are sections of DNA ...
14
votes
3answers
1k views

Is the size of the genome across species roughly the same?

Chromosome number differs across species. Is the amount of DNA comparable between organisms, just being split into smaller chunks in those species with more chromosomes? Or do species have different ...
0
votes
0answers
24 views

Is it possible that the DNA of an individual changes significantly over a period? [duplicate]

Is it possible that the DNA of an individual changes significantly over a period ?
3
votes
0answers
35 views

How stable is linear DNA transfected in eukaryotic cells?

I would like to know, with references from the literature, what is the half life of a linear dsDNA transfected in a mammalian cells. For example, if I transfect human cells with a PCR product ...
6
votes
0answers
41 views

What is the fate of micronucleus DNA?

Micronuclei are cellular structures that are formed as a by-product of, usually, defective mitosis. The piece of chromosome in a micronucleus may, or may not contain a centromere and the DNA is ...
2
votes
1answer
72 views

Consequence of Plants as Incomplete Protein Source

Some years ago, in a 1000~ level biology course we learned that the DNA essentially encodes formulas for creating proteins from amino acids. While the human body can synthesize many many amino acids, ...
7
votes
1answer
128 views

What determines the number of chromosomes an organism carries?

This is an extension of this question about What limits chromosomal length?. I am wondering what could be the specific reasons behind the number of chromosomes an organism carries. In other words, ...
0
votes
1answer
38 views

X-recessive disorder pedigree confusing me

This is a pedigree of a family in which bleeder disease, an x-recessive disease, is present. I am asked which marker, A or B, the disease is linked to. I've asked about this type of problem before: ...
0
votes
1answer
24 views

Why can't this be 1st meiotic division non-disjunction as well?

The child has gotten 2 "sick" chromosomes from his dad and 1 healthy from his mom, leaving it with trisomy 13. I can see how this can happen in the second meiotic division of the father but I can ...
0
votes
2answers
32 views

Chromosomal translocation notation

A reciprocal translocation is denoted for example: t(4;12)(q32;q24) indicating that q32 on chromosome 4 being swapped with q24 on chromosome 12 if I understand this correctly. My question is: How do ...
0
votes
1answer
28 views

X-recessive disorders and genetic markers

Please observe the following pedigree of a family with a x-recessive disease (bleeder disease). The A's are genetic markers so close to the disease gene that recombination is negligible. I ...
-1
votes
1answer
39 views

Pedigree problem and type of inheritance [closed]

Please observe the following pedigree and come up with a conclusion as to which type of inheritance is most likely Now it should be obvious that it's either autosomal dominant or autosomal ...
3
votes
1answer
62 views

Walk me through microsatellite markers and PCR

Three polymorph microsatellite markers are used to try and narrow down the location of a disease locus, with the use of PCR with 2 flanks on each side of the actual polymorphic area. The PCR ...
3
votes
1answer
40 views

Gene frequency problem

1/330 of white people in south Africa have a disease called Porphyri. What is the frequency of the gene if it is inherited autosomal recessive? This one I understand. The genotype frequency is $q^2 ...
2
votes
0answers
37 views

Write the haplotypes of the family

I'm doing old exam assignments to prepare for my finals on Monday and I've stumbled on one assignment that I'm not sure how to tackle. A family with 2 children is examined for cataracts using PCR ...
1
vote
1answer
42 views

Dynamic mutation and huntington's

I read that Huntington's is a disorder caused by dynamic mutations in the DNA, which means that a triplet sequence of DNA changes from generation to generation. Say we have the sequence ATGATGATGATG. ...
4
votes
3answers
92 views

What is a genetic marker?

In DNA sequencing and analysis, what is a genetic marker? I've heard that microsatellites are genetic markers? Those are repetitive strands of bases such as GCAGCAGCAGCA etc. Why are they markers and ...
2
votes
1answer
81 views

From a computer science perspective, how is DNA compared for various purposes?

I am very interested in privacy preserving technolgies, such as Microsoft PINQ and would like to see if this is applicable to DNA comparison. Given that I don't have a background in biology, I ...
2
votes
0answers
53 views

CRISPR-Cas Systems

In the context of the bacterial systems (not the gene editing tool), I was wondering what happens to the foreign DNA after the Cas proteins have created a new spacer. It is really not clear to me, ...
0
votes
0answers
48 views

Can these “Promega Wizard genomic DNA Purification kit” be used for samples not stated directly?

I am not sure if this is a duplicate of my previous question, it is somewhat related, so sorry if I misunderstood. The question is, can i use the above kit for samples like hair, fingernails, etc? ...
1
vote
1answer
83 views

DNA extraction methods for hair?

TLDR: Can anyone state a extraction and isolation method(s) for genomic DNA for hair that will be used for PCR, in detail is preferable since I am a novice. I tried googling for DNA extraction ...
3
votes
1answer
113 views

Restriction sites

I would like to know: how many restriction sites does a restriction enzyme use on a DNA molecule? In other words: If a sequence on a plasmid contains the following bases: ATTGCAGTCTG and I want ...
4
votes
0answers
81 views

Do DNA supply houses maintain a watch list for dangerous oligos (Anthrax, Smallpox, etc.)?

The recent work by DeLoache, et al. on a synthetic opiate-precursor production pathway in yeast has generated a lot of scare stories in the media about people homebrewing heroin as easily as they ...
2
votes
0answers
68 views

Can DNA test of my grandparent's brother reveal my heritage from that branch of the family?

I'm thinking about doing that DNA test on all my grandparents to know where their genes come from. They are getting very old and I don't want to let the opportunity pass to gather more knowledge about ...