Tagged Questions
1
vote
3answers
160 views
Separating DNA Fragments by Gel Electrophoresis. Are all the strands for one size the same?
My apologies if my question is too basic, and please point me to a more appropriate forum. I am reading the textbook "Essential Cell Biology" by Alberts et al, and am consulting other sources as ...
3
votes
1answer
99 views
What is the DNA/protein charge ratio?
To study DNA-protein interaction, I want to do a DNA retardation test by mixing the protein with DNA and afterwarts loading it on an agarose gel to see if the DNA migrates slower. I've found some ...
12
votes
3answers
3k views
How many agarose gel bands are typical for circularised DNA
I am aware that circular DNA can be both relaxed and super coiled. However when running an agarose gel of the circular plasmid along with singly digested plasmid with BamHI and HindIII, I see 1 band ...
