A structural aberrancy in DNA, as opposed to a sequence change (mutation). Often caused by reactive species.

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RF Cautery DNA damage?

I'm concerned about the safety and mechanism of RF cautery. The frequency of the radio waves used is 4Mhz so normally there should not be any DNA damage BUT the thing you read everywhere is that only ...
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46 views

How can we create a living dinosaur using DNA technology?

I'm wondering what would we need to do to create a living dinosaur using DNA technology? If it is not possible with current technology, will it ever be feasible? In the movie Jurassic Park, ...
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Questions on DNA damage

I'm not strong in biology, so bear with me on this: I've been reading that as we age, our DNA is damaged by internal (e.g. errors during replication) and external (e.g. sun damage or radiation) ...
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275 views

Why AZT is selective towards HIV and doesn't impair human DNA replication?

I've found this article, which is a very old one (from the time when nucleoside analogs where researched as a possible way to prevent replication of virus genetic material, before the HIV epidemics). ...
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50 views

Repair wrong DNA pair after ending replication?

If there are wrong DNA pair, for example A=G, is there mechanism that could repair such things - after the replication was finished? Or it happens only during replication?
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38 views

The damage of cancer cells

I read about the molecular biology of cancer, and I have a mess on my head and a lot of questions.. . My primary question is- The damage of the cancer cells is in the dna sequence or in the gene ...
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136 views

How does DNA damage cause ageing in yeast?

As I understand it, in yeast ageing there is daughter cell and mother cell. The daughter cell is has newly "fresh" DNA and mother cell dies after some counts of replication. What happens to the ...
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G>T transversion VS. T>G transversion?

So I'm reading about how mutations in DNA can be caused by oxidative damage. An example of a product of oxidative damage is given: 8-oxo-7-hydrodeoxyguanosine My textbook says that this product ...
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249 views

How does non-homologous end joining (NHEJ) work?

I was reading about non-homologous end joining (NHEJ) in my molecular biology of the gene textbook but the explanation provided in the text was rather vague to me, and I was not able to understand it ...
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How do nicks in the DNA strand affect the success of Long Range PCR?

Long Range PCR using NEB Master Mix - Hot Start Taq was working fine for me (amplicon sizes of ~10kb) but stopped working all of a sudden. Is it possible that many freeze-thaw cycles on the DNA ...
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115 views

Question about the DNA glycosylase enzyme assay/formamide gel loading buffer

Often for the DNA glycosylase enzyme assay we can see that the DNA glycosylase is incubated with oligonucleotide in 37C and then reaction is stopped by addition of formamide gel loading buffer (80% ...
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If everybody DNA is different then how blood can match even with matching blood group?

We know that everybody's DNA pattern is different in the world. Then how can ┬┤we transfer blood from one person to another person and this person can survive ?
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Is a single x-ray photon capable of doing enough damage to the DNA of a cell to kill that cell? [closed]

Ionizing radiation is damaging to DNA, but is there such a thing as a safe dose below which no DNA damage could occur? I'm not asking about a minimum safe dose overall, just whether or not there's an ...
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Stay young by reinserting own DNA from years ago?

As far as I know, the DNA sequence becomes shorter every time a cell divides. A shorter sequence results in information loss and aging characteristics. As a countermeasure, shouldn't one stay young ...
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154 views

Can cancer cells in the same person, organ, and origin have different DNA?

Is it possible for cells from the same tumor to have different genetic material, and if so, to what degree is it possible (how fast do they mutate) ?
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115 views

Which is the best way to fix and preserve comet assay slides before staining with Ethidium Bromide?

I want to use the method of Single Cell Gel Electrophoresis (comet assay). I have many samples, but I don't have the appropriate microscope. So I have to preserve my slides until the time comes for ...
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2k views

Difference between mutation and DNA damage

What is the strict difference between mutation and DNA damage? As far as I understand it, a mutation is an alteration in the genetic sequence, having "tricked" the repairing machinery and thus ...
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81 views

Do mitochondrial DNA have repair enzymes?

Mitochondria are considered endosymbionts originated from a prokaryote. So, do they have a DNA repair mechanism ?