3
votes
1answer
15 views

How do nicks in the DNA strand affect the success of Long Range PCR?

Long Range PCR using NEB Master Mix - Hot Start Taq was working fine for me (amplicon sizes of ~10kb) but stopped working all of a sudden. Is it possible that many freeze-thaw cycles on the DNA ...
1
vote
3answers
74 views

If everybody DNA is different then how blood can match even with matching blood group?

We know that everybody's DNA pattern is different in the world. Then how can ยดwe transfer blood from one person to another person and this person can survive ?
3
votes
0answers
46 views

Is a single x-ray photon capable of doing enough damage to the dna of a cell to kill that cell?

Ionizing radiation is damaging to DNA, but is there such a thing as a safe dose below which no DNA damage could occur? I'm not asking about a minimum safe dose overall, just whether or not there's an ...
2
votes
2answers
89 views

Stay young by reinserting own DNA from years ago?

As far as I know, the DNA sequence becomes shorter every time a cell divides. A shorter sequence results in information loss and aging characteristics. As a countermeasure, shouldn't one stay young ...
1
vote
1answer
75 views

Do mitochondrial DNA have repair enzymes?

Mitochondria are considered endosymbionts originated from a prokaryote. So, do they have a DNA repair mechanism ?