1
vote
2answers
18 views

How does non-homologous end joining (NHEJ) work?

I was reading about non-homologous end joining (NHEJ) in my molecular biology of the gene textbook but the explanation provided in the text was rather vague to me, and I was not able to understand it ...
3
votes
1answer
29 views

How do nicks in the DNA strand affect the success of Long Range PCR?

Long Range PCR using NEB Master Mix - Hot Start Taq was working fine for me (amplicon sizes of ~10kb) but stopped working all of a sudden. Is it possible that many freeze-thaw cycles on the DNA ...
1
vote
3answers
83 views

If everybody DNA is different then how blood can match even with matching blood group?

We know that everybody's DNA pattern is different in the world. Then how can ┬┤we transfer blood from one person to another person and this person can survive ?
3
votes
0answers
50 views

Is a single x-ray photon capable of doing enough damage to the dna of a cell to kill that cell?

Ionizing radiation is damaging to DNA, but is there such a thing as a safe dose below which no DNA damage could occur? I'm not asking about a minimum safe dose overall, just whether or not there's an ...
2
votes
2answers
92 views

Stay young by reinserting own DNA from years ago?

As far as I know, the DNA sequence becomes shorter every time a cell divides. A shorter sequence results in information loss and aging characteristics. As a countermeasure, shouldn't one stay young ...
1
vote
1answer
76 views

Do mitochondrial DNA have repair enzymes?

Mitochondria are considered endosymbionts originated from a prokaryote. So, do they have a DNA repair mechanism ?