A methyl group can be added to a cytosine residue, usually one that neighbors a guanine (known as a CpG - cytosine-phosphate-guanine - site). This can affect chromatin and histone structures, as well as directly influencing binding of various factors, thereby altering the transcription of genes.
I am looking for the names of some environmental alkylating agents (in context of DNA damages) found for example in the fume produced by industry. Most of the papers which I have found is concentrated ...
We have a long protocol that we are optimizing that includes DpnI digestion of a PCR product (to remove any of the template DNA if it's methylated, and while we're not certain in the blind tests, ...
Cytosine residues in DNA that can be methylated (i.e. CpG sites) are likely to be in the same methylation state if they are geographically (proximally) close together. I can only find one paper that ...