Tagged Questions
2
votes
0answers
22 views
What is the purpose of Y-shaped adapters in Illumina sequencing?
Y adapters different sequences to be annealed to the 5' and 3' ends of each molecule in a library.
The arms of the Y are unique, and the middle part, connected to the DNA fragment, is complementary.
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5
votes
1answer
105 views
Is there a detectable amount of bacterial DNA in the blood of infected persons?
With which bacterial infection in humans has it been shown that bacterial DNA can be found in the blood?
If any is found it is likely not to be very much, and even difficult to distinguish from ...
4
votes
2answers
89 views
Sequencing from PCR
As far as I understand it, PCR can be used to make many copies of one gene. My question is, is it possible to sequence DNA after PCR and is it easier than sequencing it via other methods. If it is ...
7
votes
1answer
298 views
How to clone and sequence a gene transcript of unknown sequence?
How might I go about amplifying a gene transcript (mRNA) from animal tissue of which little is known about the genome? In some applications, I have used reverse transcriptase PCR to amplify all mRNA ...
