Deoxyribonucleic acid (DNA) is the carrier of genetic information, including for all known living organisms. The only known exceptions are RNA viruses.

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How does one measure the length of telomeres?

My questions are: What techniques are available for doing so? Are they destructive ? What is their accuracy ?
5
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1answer
400 views

Primer Dimer / Hairpin Algorithms

What are the algorithms / methods in use for the calculation of primer dimers and hairpins? As an example, IDT's OligoAnalyzer tool will generate these analysis given particular sequences. The ...
25
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3answers
389 views

Is telomere length a reliable measure of health/lifespan?

Several companies are commercializing tests for telomere length such as this one here. I understand the basic mechanism for why telomeres shorten during DNA replication, but how good is the evidence ...
7
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3answers
120 views

Free Radicals for aging

From my understanding free radicals play a slight role in ageing. In what ways are they so damaging, and can a restricted diet reduce production of free radicals?
3
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1answer
174 views

What is the DNA/protein charge ratio?

To study DNA-protein interaction, I want to do a DNA retardation test by mixing the protein with DNA and afterwarts loading it on an agarose gel to see if the DNA migrates slower. I've found some ...
6
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1answer
138 views

Are there any DNA base sequences that are fully conserved between the genomes of all humans?

That is, they don't differ throughout the entire population. I understand of course that we can't DNA sequence every human, so by "fully" I mean there's an incredibly small probability of there being ...
3
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2answers
122 views

How distantly related are eusocial insects? Aren't members of a species much more related than 1/4, 1/2, or 3/4? [duplicate]

Possible Duplicate: How many genes do we share with our mother? I went to a lecture that talked about the behavior of social insects in terms of their relatedness of genes. For instance, ...
5
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2answers
93 views

Synthetic biology using existing cells

I was watching the video at this link: http://www.bbc.co.uk/news/science-environment-17436365 The speaker says that a cell is taken and its original DNA content is stripped out and replaced with ...
6
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1answer
323 views

How can I predict DNA binding affinities from a protein sequence?

Are there any computational tools to predict the binding affinity to specific DNA motifs from protein domain sequence information?
13
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2answers
205 views

What is the contribution of viruses to the evolution of mankind?

I'm interested in horizontal gene transfer in bacteria, viruses, and organisms such as Bdelloid Rotifers. I've just read in Carl Zimmer's 'A Planet of Viruses' the following passage: As a host ...
5
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2answers
95 views

Do somatic cells alter their own nucleotide sequence?

I seem to remember reading that embryonic cells will frequently replicate the section of their genome containing rDNA by splicing in duplicate genes. The cells use this to produce ribosomes at a rate ...
5
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2answers
172 views

Are there other mechanisms for mutation besides imperfect DNA replication?

I was reading http://www.askamathematician.com/2012/05/q-is-quantum-randomness-ever-large-enough-to-be-noticed/ and saw: [...] the evolution of entire species can be changed by a single mistake ...
3
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2answers
108 views

What risk to DNA does long-term exposure to low-dose radiation pose?

A new study from MIT scientists suggests that long-term exposure to low-radiation poses no risk of DNA damage for mice (it is also important to note that mice are unusually susceptible to cancer). So ...
9
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3answers
186 views

Does GFAJ-1 use Adenosine triarsenate as its energy currency?

Regarding the bacteria found in Mono Lake, CA that scientists believe uses or can use arsenic in its DNA backbone where life as we know it uses phosphorus (according to their experiments depriving the ...
15
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2answers
228 views

Can methylation from DNA get copied to RNA during transcription?

Methylation on gene-body and 3'UTRs if copied to mRNA can potentially regulate post-transcription modifications or expression regulation. But I'm not sure if they are maintained after transcription or ...
7
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1answer
73 views

How do nuclear receptors locate each other to form a DNA loop?

Nuclear receptors can influence transcription far up- or downstream from their own binding sites by looping DNA (Rubina et al.; J Mol Bio 2004). I am not sure how exactly the receptors first attach ...
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votes
1answer
224 views

DNA chip contains a complete set of random hexanucleotide (6-mers) probes? (Solve) [closed]

can you tell me how to find solution to this question A DNA chip contains a complete set of random hexanucleotide (6-mers) probes. Out of the 4^6 = 4096 probes, how many will form perfect ...
8
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1answer
200 views

Finding DNA from Amino Acid sequence problem

My attempt: First I took the single letter AA codes and made them amino acids. So, the first one is Trp which is 5'-UGG-3'. From this I got the DNA sequence 3'-CCA-5'. However, the correct ...
4
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1answer
273 views

Finding exons in DNA problem

My attempt: I looked for the TACs because I thought this would be AUG in mRNA and ultimately Methionine (the start codon). But apparently, that's not how you do this problem. Im confused because ...
2
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2answers
89 views

Consensus codon optimization by organism

Does a public database exist that contains this information? I'm trying to make a simple gene annotation program that will let me input a DNA sequence and then optimize it based on one of these tables ...
1
vote
1answer
88 views

Generating custom human DNA sequences based on traits such as eye colour?

I'm wondering if it would be possible to create software (unless some already exists, but I couldn't find any) to generate human DNA (the base pairs on the double helix) containing genes representing ...
9
votes
3answers
386 views

Source of DNA sequences

I'm working on a project where I am taking DNA sequences and translating the codons into musical notes. I have some good ideas of how to do this, I'm just not sure what sequences to work with. My case ...
6
votes
3answers
340 views

What's the use of DNA sequencing results?

Suppose I sequence DNA of some organism (a human perhaphs) and now I have the entire DNA "string" - the sequence of nucleotides. What's the use of that? It's just a "string" where nucleotides encode ...
26
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4answers
1k views

What exactly are computers used for in DNA sequencing?

I've thoroughly read the Wikipedia article on DNA sequencing and can't get one thing. There's some hardcore chemistry involved in the process that somehow splits the DNA and then isolates its parts. ...
9
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2answers
160 views

Are there viruses that affect many kinds of cells across species?

I'm not a professional in biology nor a student, but I'm curious about this. To be more specific: why doesn't a plant virus affect animal cells? I suspect that different kinds of cells have different ...
9
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4answers
202 views

How can I avoid digesting protein-bound DNA?

I'm interested in sequencing and analyzing the bound DNA, and minimizing the amount of unbound DNA that gets sequenced through digestion. When digesting protein-bound DNA, is all of the unbound DNA ...
10
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1answer
555 views

Why is the 3'UTR region highly methylated in most of the human genes?

Most of the human genes are found to be highly methylated in their 3'UTR region (0.8-0.9%). I was wondering if there is any specific reason for this?
12
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3answers
308 views

What is Curved DNA?

CbpA is DNA binding protein found in E. coli and binds non-specifically to curved DNA (Cosgriff et al., 2010), when the bacterium is in a static phase of growth. The use of "curved DNA" confuses me. ...
17
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2answers
2k views

Which organism has the smallest genome length?

Which animal/plant/anything has smallest length genome?
7
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1answer
811 views

Does extracted DNA degrade after a certain time period?

For direct use as template in PCR runs. Chelex 100 5-10% w/v extraction. Without listing the whole protocol, in the end the supernate is decanted off and then stored at 4°C. I was under the impression ...
6
votes
2answers
282 views

What is a good Non Fluorescent DNA Dye?

Can anyone suggest a dye which specifically targets DNA, but is not fluorescent? (We plan to mark DNA before observing it with RAMAN-Spectroscopy. Because of the weak Signal even a low emission would ...
13
votes
1answer
193 views

What is the prehistory of amino acids in cells?

As a followup to Why 20 amino acids instead of 64? and What is the smallest number of amino acids required for life?, I am trying to understand the prehistory of amino acids in cells. All living ...
6
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1answer
186 views

What is the origin of “melting” in molecular genetics?

I'm reading some papers about prokaryotic transcription mechanisms, and I've come across a term I haven't heard before: DNA melting or promoter melting. After reading a bit, it's pretty clear that ...
9
votes
1answer
1k views

Are human chromosomes connected or separate molecules?

Do the 46 human chromosomes form a single unbroken DNA helix? Or is it rather that a human's genome consists of 46 disconnected helices? If it is the former, does the common numbering scheme for ...
11
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2answers
5k views

Does an annealing temp higher than primer's Tm contribute to primer dimer?

I am attempting to reproduce results from a number of journal articles all referring to the same SNP. In doing this I'm using the same primer set outlined in the articles. When I attempted a run the ...
30
votes
2answers
1k views

How do cockroaches resist the effects of ionizing radiation?

Cockroaches are very hardy insects. It is known that, among other things, they are able to withstand bursts of ionizing radiation that would kill a human being. The explanations of this observed ...
9
votes
2answers
398 views

Do mitochondria simply automatically convert glucose to ATP?

I wonder whether there is any intelligent decision-making in mitochondria in the timing of their operation. Do they simply begin ATP production as soon as the correct reactants are present? Or, ...
5
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1answer
237 views

What is the benefit for cells having the ATP production regulated in mitochondria compared to being from the nucleus?

Mitochondria have their own DNA and appear to be loosely connected to the nucleus and it role. Why are the functions of mitochondria not in the nucleus? Why doesn't the nucleus control the ...
13
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3answers
1k views

What implications has the missing 2'-OH on the capability of DNA to form 3D structures?

The chemical difference between RNA and DNA is the missing 2'-hydroxyl group in the nucleotides that build DNA. The major effect of that change that I know of is the higher stability of DNA compared ...
12
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1answer
205 views

Abiogenesis: Beyond the research journals as a lead in to discussions on evolution

I just came across this abstract: Aminoacyl-tRNA synthetases (aaRSs) are responsible for creating the pool of correctly charged aminoacyl-tRNAs that are necessary for the translation of genetic ...
15
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6answers
878 views

Could Junk DNA be used as a Turing Machine by nature?

In what ways has DNA been studied to see if there a "programmable" aspect to it? Has nature produced anything resembling a Turing machine within the cell, perhaps using the "junk DNA" as its code? I ...
10
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1answer
167 views

How can you identify if a person is homozygous for a certain allele?

I've been thinking about starting a small private research project. In this project I need to find out whether a person is homozygous for a certain allele. The reason for this is that I'm really ...