Deoxyribonucleic acid (DNA) is the carrier of genetic information, including for all known living organisms. The only known exceptions are RNA viruses.

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book on genealogy, haplogroups, migrations, etc

Looking for a popular science book on the topic. I need a wide-angle expose, not fiddling with minor details. It should also be somehow intertwined with migration. I've recently learned, for example, ...
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How do I figure out which of the valleys I came from?

I had my DNA tested by 23andme.com. They give me a very long string for my DNA. How do I determine which of the three Ethiopian valleys I descended from? I'm of European descent. ...
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enzymes that stabilize DNA loops

As a follow-up of a previous question, I would like to know what enzymes or protein complexes have been used to manipulate DNA samples into stabilizing DNA loops. I have read that cohesin is one of ...
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Is a single x-ray photon capable of doing enough damage to the dna of a cell to kill that cell?

Ionizing radiation is damaging to DNA, but is there such a thing as a safe dose below which no DNA damage could occur? I'm not asking about a minimum safe dose overall, just whether or not there's an ...
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Gibson assembly - primer design with A and T rich regions

I have question about Gibson assembly. I have done it several times and it always worked okay for us, but now I want to assemble together a fragment which has sequence like this: ...
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Mitotic crossover happens in G1?

I was reading this article in wikipedia and came across this : It has been suggested that recombination takes place during G1, when the DNA is in its 2-strand phase, and replicated during DNA ...
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What percentage of chimeric takes up in a cDNA library?

I get a cDNA library of species of Mollusca,and complete some of the clones with polyA tails.But when I translate them with three frames,I find there is a large percentage of sequences that cannot ...
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condensed protocol for sequencing a portion of human DNA from buccal sample

Anyone have a short & sweet protocol for PCR amplifying a region of human DNA (chromosomal or mt, I don't care) extracted from a buccal sample: including validated primer sequences and preferred ...
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59 views

DNA content in seeds vs. fruit flesh

Is there a publication comparing DNA yield and / or PCR-amplifiability after extraction from fruit flesh (like apples, oranges, cherries etc) in comparison to seeds of the same fruits ? I would prefer ...
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Phylogentic Footprinting Project

I'm working on performing phylogentic footprinting to figure out how many TRE sequences there are in the TWIST1 gene. (Sequence motifs). I'm trying to use ENSEMBL to gather orthologues of species in ...
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DNA fingerprinting

I would like to make my own DNA fingerprint - just for fun to have my "autoportrait":). I was looking around a bit and all the commercial kits you can have are very expensive. Can you suggest me a ...
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Transcription takes place from the 5’ to the 3’ end of the m-RNA. Why?

Only one side of the DNA ladder is copied (the sense side). The sense side starts with a 3’ end. This means the corresponding mRNA will have to assemble starting from the 5’ end. This is my initial ...
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what are hyperbranched amplicons in DNA sequencing?

I am reading an article about single-cell sequencing: http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.2720.html And came across the concept of "hyperbranched amplicons". I googled for it but ...
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3' or 5' DNA capping protein

Does anyone know of a protein/molecule that can be attached to one end of the DNAs temporarily ? eg. i would like to only to allow one strand of DNA be ligated to another DNA molecule to either it's ...
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DIY extraction and long term storage of human DNA sample?

What is the recommended DIY way of extracting and long term storing of human DNA samples to store at home and send for sequencing in years time? Considerations: Sample material: cheek swab, hair, ...
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5,6-dihydrouracil and 5,6-dihydroxyuracil - how they arise in DNA?

5,6-dihydrouracil can be formed from cytosine after exposure of DNA to ionizing radiation under anoxic conditions [1]. What are other ways by which 5,6-dihydrouracil is formed in DNA? What about ...
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Is it possible to resurrect dead animal by using it's DNA?

My pet died yesterday by an accident. I'm feeling so sad, can't even sleep well. I tried to control myself and thought about many philosophical questions, but I can't get away from this sadness. ...
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Stress-responding enhancer?

I wonder if anyone knows a well characterized human enhancer which has been shown increased activity (in e.g. reporter assays) under stressed conditions, such as DNA damage, hypoxia, ...
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89 views

Ribosomal Turing Machines, DNA/RNA computation

I'm a computer science guy, recently crossing over to do some research in computational biology on RNA secondary structure prediction. While looking through the materials I got a crazy idea, what if ...
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Why does the stability of YAC increase with size?

From Lehninger Principles of Biochemistry : The stability of YAC increases with size (up to a limit). Those with inserts of less than 100000 are gradually lost during mitosis. Why does this ...