Questions pertaining to fruit flies of the genus *Drosophila*.

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8
votes
2answers
110 views

What are these two fruit flies doing?

When taking some pictures I accidentally captured two fruit flies doing this: The photos are unfortunately blurry, but from what I can tell, one fly approaches the other from behind, and something ...
5
votes
1answer
81 views

How many genes does D. melanogaster have?

Obviously there is no 100% exact number, but I came across this on flybase, the gold standard for annotation. I am confused now. "Genes located to the genome", is that what I am looking for? If so, ...
2
votes
1answer
22 views

Toll Like Receptors Vs Toll Receptors

What are the major differences between them, apart from one being in humans and other in Drosophilla?
1
vote
1answer
37 views

Disadvantage of using Drosophila for breeding experiment

What should be the disadvantage of using Drosophila for experiments? a)small size of larva. b)short life cycle. c)mating soon after emergence of flies. d)large number of offsprings. Options (b) and ...
2
votes
1answer
73 views

Through which mechanisms did the Drosophila Histone Cluster evolve?

The Drosophila Histone Cluster is a gene cluster that contains more than a hundred copies of a sequence that encodes several histone proteins. These copies are very similar. My question is what ...
2
votes
0answers
45 views

How do sex biased genes evolve?

I am wondering how do genes become sex-biased? that is, how does a gene evolve expression which is regulated in a sex-specific manner (assuming no effect from sex-limited Y/W chromosomes). I ...
4
votes
1answer
283 views

Measuring body size in flies/insects

"Mesothorax length (the distance from the tip of the scutellum to the most anterior part of the mesothorax)" From Bergland et al 2008. Would that be this distance (green line)? If not, how ...
1
vote
0answers
54 views

Drosophila biarmipes gene annotation, transposable element?

When doing an annotation of a contig31 of Drosophila biarmipes genome, the gene predictions(in orange, red, green, tan, blue, brown, and gray) picked up numerous possible genes in the area circled in ...
7
votes
1answer
91 views

Chromosome picture, how to interpret?

In this guide i was reading for annotations, there is a diagram of chromosomes of D.mel, I am confused as to why the chromosomes are all attached to one another, is this picture taken in a certain ...
3
votes
0answers
57 views

Degree of dosage compensation in Drosophila melanogaster

In this paper the authors state that the dosage compensation seen in Drosophila is approximately twofold, but they do not provide any source or numbers (as far as I can see) for this. What is the mean ...
1
vote
0answers
32 views

Is recombination suppressed in the males of all Drosophila species? Any other species?

Following up from this question, I am wondering if the phenomenon of genome-wide suppressed male recombination is limited to Drosophila melanogaster or is common to all Drosophila? The answer to the ...
2
votes
1answer
869 views

Crossing white eyed Drosophila with sepia eye

So, I'm doing some drosophila experiments with my high schoolers and I had really poor production of wild type individuals for doing the experiment resulting in not enough to cross with everyone... I ...
6
votes
1answer
279 views

How much total RNA can be extracted from Drosophila brain

I am wondering how much total RNA could be extracted from a single D. melanogaster brain. I could not find this information from the literature. The closest hit was this paper, that claims that ...
6
votes
1answer
80 views

Can microdialysis be made in Drosophila melanogaster?

I've asked this before in stack overflow's cognitive science community, and someone recommended me to ask here: I've found a couple of studies using microdialysis on insects, but didn't found any in ...
3
votes
1answer
96 views

Looking for protocol to produce a drosophila primary cell line

I'm looking for a general (and easy, this is going to be for an undergrad class) protocol to isolate and culture primary cells from fruit flies.
1
vote
2answers
137 views

Heterochromatin binding sites position information

I am working on a project and I'm trying to find data on the position of genes and heterochromatin binding sites such as HP1 in Drosophila melanogaster. Is this information available for the DGRP ...
14
votes
2answers
433 views

Have we ever observed two drosophila lineages that evolved reproductive isolation in labs?

Background The standard definition of species refers to the concept of reproductive isolation. If two lineages are found to be reproductively isolated, then we consider these two lineages to belong ...
1
vote
1answer
39 views

Drosophila Crosses

I have 2 transgenic lines of Drosophila. I would like to cross them and get both transgene in the same fly. To start this cross, does it matter which fly is female or male? Or as long as the female is ...
3
votes
1answer
189 views

Drosophila melanogaster eclosion

Is there any published data which shows the number of Drosophila melanogaster fruit flies eclosing (hatching out of the pupae) against time since egg oviposition? I'm thinking there should be ...
1
vote
1answer
157 views

Studying the fruitfly nervous system

I'm studying Neuroscience and Fruit Flies are a pretty big deal but I don't know as much as I think I should about them. Are there and comprehensive sources that I could read? I'd like a review of ...
6
votes
2answers
193 views

Measuring fitness / lifetime reproductive success (LRS) in Drosophila

I am planning a fitness assay of Drosophila melanogaster. I'd like to get a good measure of lifetime reproductive success (lrs) but I don't want to count all the offspring produced over a lifetime by ...
3
votes
1answer
61 views

Non-fatal / low-harm measurement of Drosophila traits

I am trying to generate some candidate traits to measure in a fitness assay of wildtype outbred (lab population) flies. The key trait I will measure is lifespan. I am looking for some additional ...
3
votes
1answer
85 views

What makes drosophila eyes red? and is it stable?

I have Drosophila melanogaster which I am doing an eye pigmentation assay on in the future. To do this I will dissolve the heads, 10 of them removed from frozen whole flies, in acidified ethanol for ...
3
votes
0answers
60 views

What are polytene bands, and why are they there?

Drosophila polytene chromosomes have been particularly useful in genetic research, as it made cytogenetic gene mapping possible with very little effort. This was primarily accomplished due to polytene ...
4
votes
1answer
345 views

Software for counting fly eggs

Is there any software that could be used to rapidly (quicker than humans) count the eggs laid by Drosophila on a substrate by placing them under a microscope with a camera attached? The eggs are laid ...
4
votes
2answers
103 views

Combining gene expression data from two species

I currently have two sets of gene expression data. The first is a dataframe of genes identified by Annotation id CG numbers (for example "CG10005") in one column and a numerical variable of interest ...
8
votes
3answers
7k views

Why doesn't recombination occur in male Drosophila?

"Males do not show meiotic recombination, facilitating genetic studies." For a while I have known that this phenomenon occurs, this quote comes from the Wikipedia page on Drosophila melanogaster, ...