Questions pertaining to fruit flies of the genus *Drosophila*.

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4
votes
1answer
218 views

Software for counting fly eggs

Is there any software that could be used to rapidly (quicker than humans) count the eggs laid by Drosophila on a substrate by placing them under a microscope with a camera attached? The eggs are laid ...
0
votes
0answers
11 views

More vectors for inducing neurodegeneration (reduction in synaptic plasticity) in D.melanogaster

I've just finished a project on loss of synaptic plasticity in Drosophila melanogaster larvae with uas control gal4 sensor for cAMP expressed in the motor neurons. I induced neurodegeneration through ...
4
votes
0answers
49 views

Can microdialysis be made in Drosophila melanogaster?

I've asked this before in stack overflow's cognitive science community, and someone recommended me to ask here: I've found a couple of studies using microdialysis on insects, but didn't found any in ...
3
votes
1answer
57 views

Looking for protocol to produce a drosophila primary cell line

I'm looking for a general (and easy, this is going to be for an undergrad class) protocol to isolate and culture primary cells from fruit flies.
10
votes
2answers
284 views

Have we ever observed two drosophila lineages that evolved reproductive isolation in labs?

Background The standard definition of species refers to the concept of reproductive isolation. If two lineages are found to be reproductively isolated, then we consider these two lineages to belong ...
1
vote
2answers
108 views

Heterochromatin binding sites position information

I am working on a project and I'm trying to find data on the position of genes and heterochromatin binding sites such as HP1 in Drosophila melanogaster. Is this information available for the DGRP ...
5
votes
2answers
112 views

Measuring fitness / lifetime reproductive success (LRS) in Drosophila

I am planning a fitness assay of Drosophila melanogaster. I'd like to get a good measure of lifetime reproductive success (lrs) but I don't want to count all the offspring produced over a lifetime by ...
3
votes
1answer
56 views

Non-fatal / low-harm measurement of Drosophila traits

I am trying to generate some candidate traits to measure in a fitness assay of wildtype outbred (lab population) flies. The key trait I will measure is lifespan. I am looking for some additional ...
1
vote
1answer
104 views

Studying the fruitfly nervous system

I'm studying Neuroscience and Fruit Flies are a pretty big deal but I don't know as much as I think I should about them. Are there and comprehensive sources that I could read? I'd like a review of ...
8
votes
3answers
3k views

Why doesn't recombination occur in male Drosophila?

"Males do not show meiotic recombination, facilitating genetic studies." For a while I have known that this phenomenon occurs, this quote comes from the Wikipedia page on Drosophila melanogaster, ...
1
vote
1answer
31 views

Drosophila Crosses

I have 2 transgenic lines of Drosophila. I would like to cross them and get both transgene in the same fly. To start this cross, does it matter which fly is female or male? Or as long as the female is ...
3
votes
1answer
106 views

Drosophila melanogaster eclosion

Is there any published data which shows the number of Drosophila melanogaster fruit flies eclosing (hatching out of the pupae) against time since egg oviposition? I'm thinking there should be ...
3
votes
1answer
62 views

What makes drosophila eyes red? and is it stable?

I have Drosophila melanogaster which I am doing an eye pigmentation assay on in the future. To do this I will dissolve the heads, 10 of them removed from frozen whole flies, in acidified ethanol for ...
3
votes
0answers
55 views

What are polytene bands, and why are they there?

Drosophila polytene chromosomes have been particularly useful in genetic research, as it made cytogenetic gene mapping possible with very little effort. This was primarily accomplished due to polytene ...
4
votes
2answers
92 views

Combining gene expression data from two species

I currently have two sets of gene expression data. The first is a dataframe of genes identified by Annotation id CG numbers (for example "CG10005") in one column and a numerical variable of interest ...