I guess the answer is about indirect one giving less error due to selectivity but how exactly does that happen?
I'm doing some ELISA development, and I'd like some justification/best practices for background correction. I'm using a horseradish-peroxidase (HRP) detection system along with a TMB substrate and an ...
What methods can be used to determine whether they are authentic or fake?
Background: There are many methods to extract proteins form human tissues out there. The majority of them use an extraction buffer containing variable concentrations of detergents and protease ...
What would be the best design for spike-and-recovery and linearity-of-dilution validation experiments in one 96-well ELISA plate?
I have searched in the web for a detailed explanation of doing such validation experiment, but unfortunately couldn't find a satisfactory one. I came across the following sources: Thermo ...