Enzyme kinetics are the studies of the biophysical properties of enzymes and their substrates.

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Enzyme Assay - pectinase

During assaying an enzyme at high temperature, the substrate (Pectin) is degraded by the high temperature rather than by enzyme, so, how can I minimize degradation of the substrate by the temperature? ...
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What's the difference between Ki and IC50?

I have just read this post at researchgate: http://www.researchgate.net/post/How_to_calculate_experimental_binding_free_energy_from_the_IC50_value I would not be very sure about what the difference ...