Enzymes are globular proteins that catalyze a biochemical reaction, increasing the overall rate by reducing activation energy. Most chemical reactions in a cell need enzymes in order to occur at rates sufficient to sustain life.

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Can an adult without genetic lactase persistence still develop a tolerance for dairy foods?

While investigating the rise of adult lactose tolerance, I came across the news that China has been encouraging its citizens to drink more milk, even though most of the Asian population lacks the SNP ...
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238 views

How do the pharmacodynamics of the NSAIDs differ and are there “resistant” COX phenotypes?

I know that the non-steroidal anti-inflammatory drugs (e.g., aspirin, ibuprofen, naproxen) affect the enzymes cyclooxygenase (types I and II). Is there any difference in the degree to which these ...
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Is the EC50 of an activating protein for an enzyme a good indicator for the binding affinity Kd?

We work with a membrane protein system where measuring the affinity between the enzyme and the upstream activating protein has been difficult, and when measured in detergent solution, it is almost 100 ...
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60 views

Pectinase Enzyme Assay

I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the ...