Organisms whose cells contain organelles, complex structures enclosed within membranes, in particular a nucleus.

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Oxygen requirements of eukaryotes

Is any of you aware of any resources where I can find information about the oxygen requirement for growth of eukaryotes? As an example, say I'd like to know whether Candida dubliniensis, ...
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What is most simple eukaryotic genome?

Expressed in number of Base Pairs or Bytes, about how large is the simplest eukaryotic genome? How much of this is 'junk-DNA' (non-coding)?
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Are HeLa cells edible?

I'm curious if HeLa cells are intrinsically poisonous or dangerous to ingest. My understanding is that some of the contamination in HeLa cells such as HPVs are not readily expressed. I have no plans ...
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Asexual reproduction and Telomeres

Many eukaryotic organisms like yeasts, hydras , planarias, plants etc reproduce asexually. Replication of End of linear DNA pose a limit to the number of cell divisions. My question : Do asexually ...
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The Origin of Mitochondria

For a long time I've just accepted, because it is just what everyone told me, that mitochondria became organelles in the cell when they were "engulfed" by another cell which acted like it's host. This ...
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Is episome a plasmid or a virus?

A plasmid is a small DNA molecule that is physically separate from, and can replicate independently of, chromosomal DNA within a cell. In general, in eukaryotes, episomes are closed circular DNA ...
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What is the best way to express two proteins in a mammalian cell?

I have two proteins and I will be preparing a vector with both genes for stable transfection. Each protein will have their own promoter and I will use piggyBac vector to insert a single cassette with ...
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206 views

Does GTP-γS (GTP gamma S) bind all GTP-binding proteins?

I've just read an article Rab10 GTPase regulates ER dynamics and morphology - Nature Cell Biology 15, 169–178 (2013) doi:10.1038/ncb2647. In this paper, to identify Rab proteins in ER, first they ...
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Do any cells change in size or mass as mammals grow?

That is to say, are there cells that, between infancy and adulthood, get larger? Or is all growth done entirely via cell division? I'm wondering if it is safe to assume that the approximate number ...
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347 views

Do adipose cells divide in adults?

I have a dim recollection of having heard that when humans gain weight, adipose cells just get larger, rather than dividing. True?
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Methods of nuclear transfection - nuclear transport

I am reading through the ENCODE papers, which is taking me well out of my comfort zone in terms of modern laboratory techniques. At the risk of asking a question which may well be thoroughly answered ...
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345 views

Why aren't mitochondria and plastids considered symbiotes of eukaryotic cells?

Mitochondria and plastids have their own DNA, their own membranes, and their reproduction is not tied to the reproductive cycle of the host cell. However, they are considered to be organelles rather ...
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What metabolically happens when an egg fuses with the nucleus of a somatic cell

In stem cell biology, it is recognized that embryonic stem cells are transcriptionally inactive for the first 3 days of development. However, during somatic cell nuclear transfer, the nucleus is ...
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238 views

What is the benefit for cells having the ATP production regulated in mitochondria compared to being from the nucleus?

Mitochondria have their own DNA and appear to be loosely connected to the nucleus and it role. Why are the functions of mitochondria not in the nucleus? Why doesn't the nucleus control the ...
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Overlapping genetic information in eukaryotes

In my research, I look at a lot of gene predictions / annotations. Frequently, I see loci where multiple gene models overlap. I haven't taken a systematic approach to analyzing these cases, but I do ...
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969 views

How does the Golgi Apparatus perform its function?

The Golgi Apparatus is an organelle with a great number of functions, I am particularly intrigued in the role it plays in packaging macromolecules and sending them to their target organelle or to ...