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3
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0answers
2k views

Experiment to show that carbon dioxide is needed for photosynthesis

In my school test, the question is as follows: Will the experiment work satisfactorily? What alterations can you make to obtain expected result? What I wrote: Yes, it will work satisfactorily. ...
1
vote
1answer
55 views

Was the Okazaki fragment experiment ever replicated?

Prof said that the experiment "proving" the existence of Okazaki fragments was never replicated. Is this true? I tried looking on Google Scholar and I cannot find any experiments talking ...
1
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0answers
24 views

Unsure why Synechococcus moves at angle in cuvette

I'd really appreciate your help. I'm working with Synechococcus. In my experiment, I have Synechococcus in a solution of milli-Q water. I illuminated it with a focused white light through a pinhole ...
2
votes
1answer
316 views

Which wavelength to use to determine algae using a colorimeter?

I am doing a Biology project involving the measurements of the density of algae (Nannochloropsis) using absorbance colorimetry. What wavelength should I set my colorimeter to? The issue is that there ...
3
votes
0answers
34 views

How to quantify the Abundance of a particular gene in Bacteria and Archaea?

For 16s rDNA metagenomic analysis, do I need to do the following steps? DNA extraction-> Purification-> PCR amplification-> Clone -> Pick every single colony-> PCR amplify -> Sequence -> ...
4
votes
1answer
95 views

What microscope/magnification would I need to observe P. Acnes bacteria?

I am currently attempting to grow a culture of P. Acnes bacteria. Right now, my only hope in identifying colonies of the bacteria in the culture is to use a black light to find colonies that glow ...
2
votes
1answer
67 views

Suggestions for self-studying experimental techniques

I would appreciate any suggestion regarding self-studying experimental techniques used in biology for a theoretical scientist (candidate). Maybe I should be more specific. I'm a biology major, but ...
1
vote
1answer
36 views

Can we directly stimulate M cones in retina with current technology?

I wonder if we could try to see imaginary "supergreen" color by directly stimulating M cones. For this I suppose we'd need: Non-destructively scan specific human's retina to locate exact positions ...
4
votes
2answers
114 views

Short, concise, practical manual for doing experimental biology

I am am physical scientist working in biology, and have recently started doing experiments. I would like a manual akin to "Numerical Recipes", but for the lab: straight forward, easy instructions on ...
4
votes
2answers
209 views

Transmembrane Protein Problem

Problem A transmembrane protein has 1000 aa. The 5th aa is found on the external side of the cell membrane. It interacts with the aqueous environment outside the cell. Amino acid 90 is inside the ...
2
votes
1answer
520 views

What causes skewed ladder bands on an agarose gel?

I frequently ran into an issue where the bands of my ladder become skewed. What causes this? Here's an example: 0.7% agarose gel, 100V, 60 minutes. Same problem occurs with 1%. I loaded 10 ul of a 20 ...
6
votes
1answer
837 views

Alfred Jost's rabbit experiment: how did he actually do it?

I was watching a Crash Course on Biology YouTube video by Hank Green: Great Glands - Your Endocrine System. Hank humorously describes a sex determination experiment by Alfred Jost as follows ...
8
votes
1answer
402 views

How should I feed and keep an ant queen?

I have captured wandering ant queen. I guess she was just fertilised and was looking for a place to start a hive. I'd like to create my own ant hive in artificial environment. I unfortunately only ...
3
votes
0answers
95 views

What is this red liquid produced by a fungal colony?

In a jar, where I had some orchid plants, a fungi start growing few days ago. I removed the plants and let the fungi to expand and grow more. After some days, red drops started to appear on the ...
3
votes
1answer
151 views

Appropriate statistical test for a student lab?

I am a HS Bio teacher and doing a microevolution lab involving candy. Essentially students use four candy types and push them together until one cracks (Nat Sel). They also do simulations of ...