Occasionally I will come across articles that refer to back-crossing mice of one strain onto the background other another strain (e.g., C57Bl6). They do not explicitly state the purpose for doing so, ...
I am computer scientist working on machine learning methods applied to predicting missing Gene Ontology annotations. In many papers, I wrote that my computational methods are very useful to suggest ...
I am struggling to find out if randomization is used in traditional genetic breeding experimentation with model organisms, especially in lab contexts? And why this should be so? This is part of a ...
I'm trying to design a primer for Gibson assembly. My gene of interest is on a plasmid, and I want to copy that gene, and put it into a different plasmid. I am unsure how to design my primers for ...
I'm reading this fantastic article on estimating body time: Molecular-timetable methods for detection of body time and rhythm disorders from single-time-point genome-wide expression profiles and one ...
I am trying to understand the assay for iPS cells in the Takahashi & Yamanaka 2006 paper. They inserted a beta-geo cassette, which contains the neomycin resistance gene, into the Fbx15 gene. The ...