Tagged Questions
5
votes
3answers
99 views
Measuring reverse transcriptase activity in E. coli
Goal: To quantify the HIV-1 reverse transcriptase (RT) activity for a number of mutated RTs.
Idea:
Put RT into a vector in E.coli.
Deliver GFP mRNA to E.coli.
RT will process the mRNA.
The ...
16
votes
2answers
147 views
What is the reason behind choosing the reporter gene when experimenting on your gene of interest?
I noticed within example experiments in class that different reporter genes are chosen to be inserted near your gene of interest to prove whether or not the gene is being expressed. For example, you ...
