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How prevalent is Taq polymerase in adding 3' A overhangs to the PCR product?
I am conducting a mutagenesis on a gene in vivo of which I need to ligate into an expression vector. The primers I have designed overlap restriction sites of which I plan to use to ligate into the ...
Apr 2 '13 at 19:07
newest experimental-design pcr questions feed
Please welcome jmac, Community Manager of the Rising Sun
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