Questions on how to plan an experiment to gain the best results. Also suitable for questions on why other researchers followed a certain procedure.

learn more… | top users | synonyms

2
votes
1answer
34 views

Can we knock out Caspase-9 *and* avoid breast cancer phenotype in our mouse model?

I am trying to design a wet lab experiment with no wet lab experience to name. Right now, in my dream land, it would be excellent if it were possible to create a Caspase-9 knockout mouse (damage to ...
3
votes
0answers
31 views

Can't resolve protein with native PAGE

This is a native gel. Let's call the left 2 lanes protein A and the right 2 lanes protein B. B is the same as A except it has a FLAG tag. They are both homotetramers of about 65 kDa. After ...
3
votes
0answers
1k views

What are the factors to consider when determining the sample size for a given study?

Say I'm designing a retrospective study (where I'd collect data from various schools for the last 5 years) to see if consuming milk can contribute to childhood obesity. How do I decide on how many ...
1
vote
0answers
33 views

Does antibody staining / immunolabeling block or inhibit protein function?

In dissociated cells, does staining them with an antibody block the protein's function? How can one assess if the protein is still functional?
1
vote
0answers
11 views

How were the synaptic areas identified in the somatic sensation pathways?

I'm studying the somatic sensation pathways in neuro-anatomy and I was wondering what was the experimental procedure for identifying the number and location of the synapses in these pathways? ...