Questions on how to plan an experiment to gain the best results. Also suitable for questions on why other researchers followed a certain procedure.

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3
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1answer
463 views

Storing Taqman plates with polymerase in fridge before running

I'm going to be running some Taqman assays on cDNA generated from RNA collected from various samples, and will end up running a bunch of plates (right now the setup is 1/2 a plate per sample, ...
1
vote
1answer
46 views

How to measure the biomass of hydroponic plants in netpot/rockwool DWC system?

I'm doing an experiment with 4 kale plants, each in their own small deep water culture (DWC) system. Each plant was transplanted in rockwool with netpots and clay pebbles. But I need to periodically ...
3
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0answers
21 views

What techniques can be used to link environmental conditions to crop yield?

Im interested in how we can determine what are the optimal conditions for crops and how deviations from these optimal conditions affect yield. In the literature I have found two main approaches and I ...
3
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0answers
409 views

Can't resolve protein with native PAGE

This is a native gel. Let's call the left 2 lanes protein A and the right 2 lanes protein B. B is the same as A except it has a FLAG tag. They are both homotetramers of about 65 kDa. After ...
3
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0answers
2k views

What are the factors to consider when determining the sample size for a given study?

Say I'm designing a retrospective study (where I'd collect data from various schools for the last 5 years) to see if consuming milk can contribute to childhood obesity. How do I decide on how many ...
2
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0answers
12 views

Does level of bats activity differs from day to day?

Say we measure bats activity as number of passes through given area. Can it differ significantly depending from day to day or it stays on average roughly the same? I am wondering whether the following ...
2
votes
0answers
106 views

Does antibody staining / immunolabeling block or inhibit protein function?

In dissociated live cells, does staining them with an antibody block the protein's function? I am sorting cells based on their expression of a few marker genes, and culturing them post-sort for ...
1
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0answers
44 views

How can I calculate Shannon-Wiener diversity index in caves with different kind of habitats and different sampling methods?

I would like to start a monitoring in different caves, compare caves of different age and length, and find out if there are any correlations between these factors and their diversity. I was thinking ...
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0answers
12 views

How were the synaptic areas identified in the somatic sensation pathways?

I'm studying the somatic sensation pathways in neuro-anatomy and I was wondering what was the experimental procedure for identifying the number and location of the synapses in these pathways? ...
0
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0answers
22 views

Determining hyaluronic acid hydrolysis via dye-binding assay

I have a protein that I am reasonably sure hydrolyzes hyaluronic acid, and I'm trying to design an experiment to test that. I was told that a dye binding plate assay where clear zones will indicate ...