Questions on how to plan an experiment to gain the best results. Also suitable for questions on why other researchers followed a certain procedure.

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What are the factors to consider when determining the sample size for a given study?

Say I'm designing a retrospective study (where I'd collect data from various schools for the last 5 years) to see if consuming milk can contribute to childhood obesity. How do I decide on how many ...
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36 views

How can oxygen production during photosynthesis be measured?

I'm curious how people measure quantitatively photosynthetic rate in labs. I've seen many qualitative examples with direct observations of bubbles forming in water due to photosynthetic output of ...
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How were the synaptic areas identified in the somatic sensation pathways?

I'm studying the somatic sensation pathways in neuro-anatomy and I was wondering what was the experimental procedure for identifying the number and location of the synapses in these pathways? ...
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36 views

Are there mice pedometers?

I am seeking to make treatments for arthritis. Earlier, I asked a question about Protocol for measuring stamina of arthritic mice, and I also know that some earlier studies have used pedometers. As a ...
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33 views

Randomized experimental design in genetic experimentation.

I am struggling to find out if randomization is used in traditional genetic breeding experimentation with model organisms, especially in lab contexts? And why this should be so? This is part of a ...
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68 views

When should endocytosis inhibitors be used in cell binding assays?

I'm beginning to do some cell-binding assays and I would like for my proteins to not be endocytosed by my mammalian cells. Typical suggestions are for the cells to be kept on ice and that the binding ...
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20 views

What models are used for the different pathways of apoptosis?

We are trying to find references for experimental models of different pathways of apoptosis (caspase-dependent and -independent intrinsic apoptosis, death receptor type-1 and -2 mediated apoptosis, ...
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28 views

Degenerate primer design for DIG in situ hybridization

New to molecular and have learned to design primers from google/youtube so any info would help Would someone be willing to share their protocol for degenerate primer design? Breakdown: Trying to ...
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50 views

what's an ethics-friendly way to measure the protocol of electric shocking mice?

We have to measure the protocol of electric shocking mice. The mice are head-fixed, we have to guarantee that the electric current is significant to make the mice learn, but not too big to make the ...