Questions on how to plan an experiment to gain the best results. Also suitable for questions on why other researchers followed a certain procedure.
3
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0answers
49 views
What are the factors to consider when determining the sample size for a given study?
Say I'm designing a retrospective study (where I'd collect data from various schools for the last 5 years) to see if consuming milk can contribute to childhood obesity. How do I decide on how many ...
2
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0answers
32 views
Where to put the gene after eukaryotic promoter for best expression levels?
As far as I know there is an optimum distance between a promoter and the gene for the best expression levels. What is that distance for common promoters like CMV, SV40? If you have a first hand ...
1
vote
0answers
50 views
When should endocytosis inhibitors be used in cell binding assays?
I'm beginning to do some cell-binding assays and I would like for my proteins to not be endocytosed by my mammalian cells. Typical suggestions are for the cells to be kept on ice and that the binding ...
0
votes
0answers
26 views
what's an ethics-friendly way to measure the protocol of electric shocking mice?
We have to measure the protocol of electric shocking mice. The mice are head-fixed, we have to guarantee that the electric current is significant to make the mice learn, but not too big to make the ...
