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2
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0answers
86 views

Can flow cytometric analysis differentiate between a parent contributing longer chromosomes versus multiple smaller chromosomes?

For flowering plants, can flow cytometry of seed from muti-generational interaction of two species and their offspring help us decide if wide differences in genome size for the two species and their ...
1
vote
0answers
39 views

Differing Flow Cytometry Genome Sizes and Sexual Mating Implies Differing Chromosome Counts

Do the fundamental principles of genetics strongly suggest the following is a derived conjecture? If 2 flowering plant species A and B have widely differing genome size (at a minimum a 50% ...
2
votes
1answer
29 views

Staining cells for FACS at 4 degrees or ambient temperature

I'm sorting a 293 derived line. One thing that is worrisome for me is cell viability after the sort. Usually I have been staining and washing at room temperature (on the benchtop) on a nutator. I have ...
0
votes
0answers
14 views

Jurkat single cell clonal expansion

I'm hoping to do a clonal expansion of Jurkat, expressing my construct which is currently transduced virally. From the FACS data I currently have roughly 30-40% expression in my culture but I need it ...
7
votes
1answer
254 views

Magnetic-activated cell sorting vs. FACS

When sorting cell populations it is possible to use either magnetic-activated cell sorting or fluorescence-activated cell-sorting (FACS). I am wondering when you would choose either technique and what ...
2
votes
1answer
260 views

Alternatives to commercially available flow cytometric analysis software for use in cell cycle analysis?

I know that many labs use either FlowJo or FCS Express to plot and analyze their flow data. These seem like the two standards for most labs (and I have used FlowJo before). However, my current funding ...
11
votes
3answers
14k views

Hoechst or DAPI for nuclear staining?

When is it best to use Hoechst vs. DAPI for nuclear staining? They seem to be very similar on paper. Are there situations where one is clearly preferable?
3
votes
0answers
159 views

Accuracy of genome size estimation by flow cytometry

I'm working on a genome project and using an in silico k-mer analysis to estimate the size of our genome based on the available Illumina reads. The k-mer based estimate is consistent across a wide ...
5
votes
2answers
896 views

Isotype control antibodies in Flow Cytometry

In a Flow Cytrometry, one can add an Isotype Control Antibody to allow another antibody to bind more specific to the cells. My question is, how can the Isotype Control Antibody add specificity to the ...
5
votes
2answers
66 views

Flow cytometry issues

I'm having problems with data analysis here. I have flow cytometry data being collected on a Fortessa, and when I import them into FlowJo 8.7, all of my fluorescence values are systematically 10X ...