A method for analyzing or purifying samples. Molecules are separated by their relative weight, charge or stereochemistry which affect their speed of movement in a gel.
5
votes
1answer
35 views
How to do western analysis to lung tissue?
I am working with lungs from mice, and I want to do a western blot analysis to my samples.
I am having trouble with this because my images turn out very "messy", with a high background.
Perhaps I ...
12
votes
3answers
2k views
How many agarose gel bands are typical for circularised DNA
I am aware that circular DNA can be both relaxed and super coiled. However when running an agarose gel of the circular plasmid along with singly digested plasmid with BamHI and HindIII, I see 1 band ...
5
votes
1answer
500 views
How do SDS-PAGE gels differ in a Bis-Tris system vs. a Tris-Glycine system?
Protein migrate differently in Bis-Tris and Tris-Glycine gels. I was curious about the actual reasons why. Do certain gel systems result in a tighter resolution than others?
