A method for analyzing or purifying samples. Molecules are separated by their relative weight, charge, or stereochemistry, which affect their speed of movement through a gel.

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What causes skewed ladder bands on an agarose gel?

I frequently ran into an issue where the bands of my ladder become skewed. What causes this? Here's an example: 0.7% agarose gel, 100V, 60 minutes. Same problem occurs with 1%. I loaded 10 ul of a 20 ...