The process by which information encoded in a gene is converted into a functional protein or RNA, resulting in or contributing to a phenotype.

learn more… | top users | synonyms

2
votes
2answers
49 views

Bioinformatics : Drawbacks of using ORA(Overlap Analysis)

What do you think are the potential drawbacks/weakness of using ORA to explain distinction between two phenotypes. I identified a few which were the dependencies of DE and the statistical method used ...
2
votes
1answer
465 views

Lyonization and X-linked disorders?

Lyonization or X-chromosome inactivation is the conversion of all but one, X-chromosomes in Females into non-coding heterochromatin (i.e. deactivated) leading to the formation of one or more Barr ...
2
votes
1answer
414 views

Optogenetics - How do microbial opsins work?

I'm just introduced to the optogenetics method and am having some trouble grasping the genetics (of the optogenteics) part of things. So we have Retinal and Opsin that form Rhodopsin molecule that ...
2
votes
1answer
100 views

What is the mechanism of regulation of PER /CRY genes?

I've read multiple descriptions of biological/circadian clocks and they all mention PER, CRY and CLOCK genes. While I kinda get how they are connected, what interests me is how these actually regulate ...
2
votes
1answer
44 views

Confusion related to the DAVID tool

I am trying to use the DAVID tool to do some gene analysis. I have some probe set intensities for some cancer cell lines. I found this link in the DAVID tool http://david.abcc.ncifcrf.gov/tools.jsp. I ...
2
votes
1answer
36 views

Epistasis when interacting loci are codons within a single gene

There is epistasis when the effect on the phenotype of one gene is influenced by one or more other genes (called modifiers). Is there a similar concept when the effect on the phenotype of one site ...
2
votes
1answer
351 views

Manipulation of gene expression using VP16 fusion and engrailed fusion to a transcription factor?

Today, a presenter briefly mentioned that gene expression in sea urchins during development might be manipulated using VP16 and engrailed fusions. On a slide, it said that expression might be ...
2
votes
1answer
293 views

Where to put the gene after eukaryotic promoter for best expression levels?

As far as I know there is an optimum distance between a promoter and the gene for the best expression levels. What is that distance for common promoters like CMV, SV40? If you have a first hand ...
2
votes
2answers
46 views

What do breeders call the effect when a breed resists modification?

It is impossible to breed a blue rose or a cat with a bulldog shape. This is because breeding is limited by gene variations in the population. What do breeders call this effect? UPDATE I guess ...
2
votes
0answers
24 views

Is it reasonable to assume that RNA polymerase would bind preferentially to the promoter of the long allele of the SERT gene?

I'm trying to come up with an idea for a school project (a hypothetical research study). I'm looking at depression and the serotonin transporter gene, which is highly expressed in the human ...
2
votes
0answers
21 views

Is it possible to make transient gene silencing by virus induced gene silencing (VIGS) in plants?

I am looking for molecular tech' which could give a transient gene silencing in plants. The objective is to not make transgenic plant, but use these tech' to silence gene of interest for a short time. ...
2
votes
0answers
158 views

which exact mechanism triggers the first cell differentiation after n divisions?

I would like to understand which mechanism triggers the first cell differentiation after n divisions. I read previous articles on SE and Wikipedia articles on cellular differentiation and ...
1
vote
2answers
73 views

Gene perturbation, what is it used for, explain to computer scientists? [closed]

I am trying to build a gene regulation network, and I need some basic knowledge of it. So, in gene research, we perturb genes. Such as knock down them? Can people tell 2 or 3 experiment methods to ...
1
vote
1answer
49 views

The reason why researchers usually use cell lines from “blast cells”?

What's the reason why researchers usually use cell lines from "blast cells" (so, immature, like lymphoblastoid cells) for measuring gene expression data? Is that they are growing up, which would make ...
1
vote
1answer
26 views

Constitutive mutation in operator gene

If a constitutive mutation happens in the operator of an inducible operon, does that mean that repressors won't be able to bind them ? Or does it mean that even if repressors are bound, they will not ...
1
vote
1answer
90 views

explanation of conjugation experiment in bacteria

In this paper (On the expression of a structural gene) I am confused about what is being plotted in Figure 6. The x-axis contains the fraction of radioactive day and y-axis contains the enzyme ...
1
vote
1answer
883 views

Why is the function of lacA (encoding thiogalactoside transacetylase) not clearly understood?

It's almost half a century since the lac operon was discovered but isn't it weird that the precise role of transacetylase isn't clearly understood ? Here a wikipedia article with a link to a journal ...
1
vote
1answer
114 views

Expression of plasmid genes

Are plasmid genes always expressed? If so, then isn't a bacterium wasting it's resources in expressing genes (like antibiotic resistance) which are not required in "normal" conditions? If not, then ...
1
vote
1answer
65 views

Genetics independent assortment?

As indicated in this question, what do you think is is the best explanation for the observed progeny, is it because distance between genes affects recombination frequency? My main concern is ...
1
vote
1answer
23 views

2 blue eyed children

My husband had light brown eyes. His father had hazel and his mother, light brown as well. His younger brother however, has blue eyes. Both my children have blue eyes. Is this possible? I thought once ...
1
vote
1answer
100 views

Shine-Dalgarno sequence and expressing proteins

Shine-Dalgarno sequence present in the prokaryotic mRNA plays a role in initiation of translation. In eukaryotes a Shine-Dalgarno like sequence is present but does not play an important role in ...
1
vote
1answer
70 views

Expression of an ancesteral gene

Why would the expression of an ancestral gene and comparing the product to a modern protein give misleading conclusions about heredity? Update: By ancestral gene I mean a gene which was used by an ...
1
vote
1answer
30 views

regarding genetic disorders related to protein production

I am not completely familiar with biology, but i had a genetics course in college along with practicals. Forgive me if there is something wrong with my question. Is there a genetic disease which ...
1
vote
1answer
16 views

Question regarding 2 constructs of tobacco plant

Six independent transformation experiments of tobacco leaf explants were carried out using two different constructs - (1) construct I containing only a Hygromycin-resistance gene $(HYG^R)$ as a ...
1
vote
1answer
57 views

Studying genetics [closed]

I am interested in studying disorders that are genetic in origin. However, I am having difficulty in understanding molecular and cellular mechanisms regarding processes such as meiosis, mitosis, and ...
1
vote
0answers
16 views

water stress expression markers in arabidopsis thaliana

So far I found papers that show studies using RNA arrays on whom they categorized water stress gene markers in root. Water stresses were reproduced by different protocols (manitol...) but always on ...
1
vote
0answers
33 views

Root hair formation in Arabidopsis

In arabidopsis, 2 cell types arise in the root epidermis : root hair cells and hairless epidermal cells. The immature epidermal cells that are in contact with 2 underlying cells of root cortex ...
1
vote
0answers
19 views

Do the phage repressors CI and Mnt exhibit crosstalk?

I am interested in using the CI and Mnt proteins as well as their respective promoters (pR and Pmnt) within the same synthetic system, but am concerned as to their similarity and potential crosstalk. ...
0
votes
4answers
160 views

Punnett Square Help

In fruit flies, red eyes are dominant over white eyes. Show a cross between two white-eye fruit flies. My question is... How do I know if the white-eye fruit flies are homozygous or heterozygous?
0
votes
2answers
107 views

How to determine the actual flux in the human metabolic model of a cell line?

The problem to be solved is to determine what the flux values are for the different reactions in the human metabolic model. As far as I understand, a good way to do that would be to use gene ...
0
votes
1answer
30 views

what is the biggest known functional distance between an enhancer and its target gene (TSS) in cis?

since the title seems self explanatory; It would be nice if anybody could provide a reference. I'm looking for a paper that I belive stated around 1.5 Mb but anything above will be cordialy welcomed. ...
0
votes
1answer
30 views

Quantitative Traits Genetics

Can someone please help with this question? I'm not so sure whether part A should be 11 inches or something else. Also, is part B correct
0
votes
1answer
33 views

Complexity in creating transgenic animals (e.g., mice)

Many papers I have seen describing transgenic rodent models (and presumably applicable to other model organisms) involve the knock-in, or modification to, a single gene, possibly two genes. With ...
0
votes
1answer
30 views

What information do microarray image convey?

OK I have read that a cell generates 4 kinds of digital (to be precise discrete) data namely DNA, RNA, Protein (sequences that can be encoded as string sequences of nucleotides/ amino acids) and micro ...
0
votes
1answer
107 views

What is the significance of and biological mechanisms demonstrated in lac operon?

I would appreciate it someone could explain clearly how the genes in the lac operon of E coli are activated to allow the bacteria to metabolize lactose? The part that I really don't understand is ...
0
votes
1answer
21 views

Known MicroRNA - Gene Systems?

Have there been any experimentally-verified systems of microRNAs targeting a gene set (e.g., in cancer, perhaps)?
0
votes
0answers
34 views

Gene linkage/mapping

I need some help with this question in the image posted. As you can see in the instructions, I'm asked to map the genes. So the first thing I did is write out the alleles corresponding to each ...