The process by which information encoded in a gene is converted into a functional protein or RNA, resulting in or contributing to a phenotype.

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617 views

Determining potential protease sites within a recombinant protein

My expressed proteins are frequently truncated and I'm trying to figure out which bands are which. The first thing to come to mind is using PeptideCutter from ExPASy but there is just a data deluge of ...
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2answers
490 views

Which is more important for protein expression mRNA structure or codon optimization?

The field seems extremely divided on the debate. On one hand, artificial experiments have suggested that synonymous mutations don't correlate with gene expression but rather, the mRNA 5' structure is ...
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1answer
253 views

Does the genetic expression of specific physical traits ever correlate to behaviour?

Over at skeptics, there were a couple questions asked as to the correlation of specific physical traits in relation to personality/behaviour. For instance, the simian line as well as red hair. Now, ...
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1answer
92 views

Time from stimulus to gene expression

My understanding is that gene expression, in response to some stimulus, generally occurs on the order of minutes. I'm curious about the extremes...the quickest and the slowest cases. What is(are) the ...
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3answers
238 views

What is the reason behind choosing the reporter gene when experimenting on your gene of interest?

I noticed within example experiments in class that different reporter genes are chosen to be inserted near your gene of interest to prove whether or not the gene is being expressed. For example, you ...