I did this experiment last month using E.coli isolated from raw milk and have following data via uv-spec: ...
In protocols for minimal media for growing bacteria I often see that a vitamin mix or a mix of various trace metals is added. But at least for E. coli I know that those are not strictly necessary, ...
Terrific Broth will result in a greater density of cells compared to LB but I have certainly seen it isn't as effective for the protein production of certain proteins compared to LB or defined media. ...
In pretty much every transformation protocol I've seen SOC medium is used to grow the bacteria for a short while after the tranformation and before plating. I've usually substituted LB medium for ...