Nutrient solution for the growth of eukariotic cells or bacteria. It can contain everything the cell needs for its growth (complete media) or can be incomplete (selective media) to select for cells that are able to synthesize specific nutrients.

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Is it essential to add cholesterol to agar or liquid culture for C. elegans?

In preparing agar plates with NGM, and also liquid cultures for growing c elegans, how essential is the addition of cholesterol and heavy metals? I have prepared both. I can't notice any obvious ...
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22 views

how to measure sugar concentration in growth medium

I am growing up bacteria in a flask and was wondering how do I track the changes of sugar concentration from the growth medium in the flask. Thanks for the help
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129 views

Can botulinum toxin be grown or kept from denaturing in an UNWRAPPED 50 pound hay bale?

Botulinum toxin is the neurotoxin protein created when botulism spores grow. The requirements for growth and/or for keeping the toxin from denaturing would seem to be very difficult to create in bale ...
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61 views

What is “bacto” peptone?

Standard recipes for yeast medium often include "bacto-peptone". Is this the same as bacteriological peptone? Is there an authoritative source that spells it out?
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220 views

Does yeast grow on LB agar plates?

I would like to grow E coli cells on cellophane on top of LB plate, and then grow yeast cells on the same LB plate. Does (or how well) yeast grow on LB plates? Is there a composition media that can be ...
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58 views

Generalism - specialism index in microbial ecology

I am doing research in Lipid metabolism in different yeast species and I would like to look into ecology of them. I would like to classify them to a Generalist/specialist based on different ...
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138 views

Can someone explain the color-changing unit (CCU) to me?

I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
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822 views

Under what circumstances and why is terrific broth preferred to LB broth for E.coli growth?

Terrific Broth will result in a greater density of cells compared to LB but I have certainly seen it isn't as effective for the protein production of certain proteins compared to LB or defined media. ...
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135 views

Growth kinetics of E.coli on media containing lactose and casein

I did this experiment last month using E.coli isolated from raw milk and have following data via uv-spec: ...
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352 views

How useful are vitamin and trace element additions in E. coli culture medium?

In protocols for minimal media for growing bacteria I often see that a vitamin mix or a mix of various trace metals is added. But at least for E. coli I know that those are not strictly necessary, ...
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Why is SOC medium recommended for transformations?

In pretty much every transformation protocol I've seen SOC medium is used to grow the bacteria for a short while after the tranformation and before plating. I've usually substituted LB medium for ...