In pretty much every transformation protocol I've seen SOC medium is used to grow the bacteria for a short while after the tranformation and before plating. I've usually substituted LB medium for ...
Terrific Broth will result in a greater density of cells compared to LB but I have certainly seen it isn't as effective for the protein production of certain proteins compared to LB or defined media. ...
In protocols for minimal media for growing bacteria I often see that a vitamin mix or a mix of various trace metals is added. But at least for E. coli I know that those are not strictly necessary, ...
I am doing research in Lipid metabolism in different yeast species and I would like to look into ecology of them. I would like to classify them to a Generalist/specialist based on different ...
I've been physically carrying out serial tenfold dilutions on samples of Ureaplasma to work out the color-changing units (CCU). As a definition, the CCU is the highest dilution at which there is a ...
I did this experiment last month using E.coli isolated from raw milk and have following data via uv-spec: ...