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Why is SOC medium recommended for transformations?
In pretty much every transformation protocol I've seen SOC medium is used to grow the bacteria for a short while after the tranformation and before plating.
I've usually substituted LB medium for ...
8
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1answer
193 views
Under what circumstances and why is terrific broth preferred to LB broth for E.coli growth?
Terrific Broth will result in a greater density of cells compared to LB but I have certainly seen it isn't as effective for the protein production of certain proteins compared to LB or defined media. ...
4
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1answer
133 views
How useful are vitamin and trace element additions in E. coli culture medium?
In protocols for minimal media for growing bacteria I often see that a vitamin mix or a mix of various trace metals is added. But at least for E. coli I know that those are not strictly necessary, ...
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Growth kinetics of E.coli on media containing lactose and casein
I did this experiment last month using E.coli isolated from raw milk and have following data via uv-spec:
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