Microanatomy of cells and tissues

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Is this a legitimate teratoma for a human pluripotent stem cell assay?

I can't quite make out this teratoma: ...
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35 views

Why can we use mouse-produced antibodies on mice tissues?

I have seen biologists use mouse grown primary antibodies in mouse tissue, and they've told me that if the blood is perfused well then there is no problem with this method. How does the secondary ...
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Peristaltic pumps in animal perfusion - how important are physiological flow parameters?

Animal perfusion is usually done according to this or very similar procedures. Though not explicitly detailed in this video, the perfusion fluid infused into the Aorta is usually driven via a ...
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90 views

Trying to differentiate between the three muscle tissues with small pictures

For an assignment I have to differentiate the three types of muscle tissues in these three pictures . I'm having difficulty seeing the striations and branches etc. because the pictures are so small. ...
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Why merkel cells are innervated by axon and not by dendrite end?

so here's the two pics that i have taken from google images and it really helped me with my question and my question is we all know that the afferent neurons receive senses for CNS which it had to be ...
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91 views

Does methanol fixation deform cultured cells?

I use methanol fixation (@ -20⁰C for 10 minutes) when performing immunofluorescence assays on cultured cells. Generally speaking, this results in very good antibody staining. However, the cells tend ...
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40 views

What are the features on a microscope one needs in order to do lab work?

By lab work I mean urinalysis, blood work(live as well), fecals, cytologies, histologies and all other. I have read(partly) a book(from 2002) on lab diagnostics and the author did not mention ...
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29 views

Where do I purchase a fly with the collagen tagged?

Where can I purchase a fly with tagged collagen? My senior undergraduate research paper is observing the collagen in larvae with normal diet compared to larvae with an experimental diet. I need ...
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88 views

Can one use a hemocytometer on a compound upright microscope?

I'm posting this as a follow-up on What to look for when buying a light microscope?. The answerer states that you would need to use a an inverted microscope to count cell in the hemocytometer(counting ...
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How many cells are there in the apical meristematic tissue?

How many cells are there in the apical meristematic tissue? Looking at this picture... , I would tend to think that there are few hundreds cells in the meristem tissue. But I guess this is a ...
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57 views

Mechanisms of bone growth

The length of a bone is caused by growth of hyaline cartilage which is then replaced by bone tissue. How do cells know whether they should grow the hyaline cartilage? What are the key molecules that ...
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235 views

What is this tissue?

Does anybody recognize this picture and can tell me what organ it came from?
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52 views

How do tight junctions in stratified squamous epithelia affect nutrient supply?

In histology we know that epithelium tissue isn't vascularized so it takes the nutrition from Lamina propia (CT) via diffusion and in other hand we know all epithilial cells have tight junction which ...
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Is epimysium same as fascia?

I have read that epimysium is dense regular connective tissue made covering the a muscle like bicep brachi. at the same time we have fascia that is made of dense regular tissue and covers muscles. so ...
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49 views

Why some neurons are tetraploid

Why are some neurons tetraploid, and how does this result from it's ancestor cell ?
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526 views

If the top epidermal layer is burned away, what is it replaced with?

If the squamous (top) layer of epidermis, or skin, is burned or damaged in another way, will it then be replaced by the next layer of below? Also, from where does the basal membrane originate? Does ...
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30 views

How many areas are there in Brodmann map?

I read Wikipedia claiming 52. I heard that this can be extended to 54. I see there is no limit of having more areas - just better understanding of the neuroblast migration and fibroblast too, ...
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215 views

Why basement membrane thickens in diabetes mellitus?

Untreated diabetes mellitus may lead to blindness and kidney failure because the basement membrane of small blood vessels in these organs thickens due to increased production of collagen and laminin. ...
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82 views

What are the internal surfaces of body cavities actually made of?

In Ross's book of histology it says that the mesothelium that includes all body cavities is made of simple squamous epithelium but in other places it says that in the gastrointestinal tract it is made ...
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122 views

what these suffixes mean,-coel,-blast,-cyst? [closed]

in the connective tissue (Bones) we have 3 types osteoblast, osteocoel,osteocyst what are they and why they differ.
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93 views

Workflow for batch analysis of ROI in histology samples

This is a pretty big task, and I have several work-arounds, but I am looking for an efficient way to go from PAS stained slides of lung tissue to defined ROI's over the whole slide (presumably via ...
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Isotropy of sarcomere bands in skeletal muscle cells

As seen under a polarization microscope, the A-Band in skeletal muscle fibres is so named because it is anisotropic in its refractive index which is a characteristic of orderly crystalline structure. ...
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31 views

How do processes of one osteocyte establish contact with processes of the adjacent cells within the mineralized matrix?

Are the processes and canaliculi formed and contacts established after the osteocytes are embeded in the matrix or are they formed during the embedding process itself. Is there any evidence to suggest ...
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100 views

Size of classical liver lobule across species

A classical liver lobule is made up of a central vein and the portal triads. A typical human liver weighs around 3 lbs. , while a typical bovine liver weighs around 12 lbs. I was wondering if anyone ...
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313 views

What is the proper quick freezing /snap freezing protocol for pancreatic tissue?

I would like to do a quick freeze on pancreas from mice. I want to then make sections (30 µm thick). The idea is to preserve a fluorescent staining done in vivo. I do not know if I should place my ...
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165 views

Significance of basal lamina for outer layers of epithelium

In stratified (not pseudostratified) epithelia such as the epidermis, what purpose does the basal lamina serve for the outer layer cells which do not even stand in contact with it? Also, how do these ...
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2k views

What is the Giemsa staining of chromosomes?

I got the question in my exam and wrote the following and I do not understand what is wrong in it: Giemsa staining is a staining method to stain particularly malaria and other parasital diseases. ...
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458 views

What is the morphological difference between Leydig cell in human and pig?

The pig is only an example, just an animal. Leydig cells have protein inclusions (Reinke crystals) that are mostly made of crystallised lipofuscin. They are secretory inclusions i.e. cells formed in ...
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208 views

What histological stain can I use for beta-keratin?

I'm trying to find a histochemical stain for beta-keratin, the type found in Reptilia which is organized in beta-sheets. It's different than alpha-keratin which is found in mammalian skin, hair, ...