Microanatomy of cells and tissues

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what these suffixes mean,-coel,-blast,-cyst? [closed]

in the connective tissue (Bones) we have 3 types osteoblast, osteocoel,osteocyst what are they and why they differ.
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Workflow for batch analysis of ROI in histology samples

This is a pretty big task, and I have several work-arounds, but I am looking for an efficient way to go from PAS stained slides of lung tissue to defined ROI's over the whole slide (presumably via ...
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812 views

Isotropy of sarcomere bands in skeletal muscle cells

As seen under a polarization microscope, the A-Band in skeletal muscle fibres is so named because it is anisotropic in its refractive index which is a characteristic of orderly crystalline structure. ...
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How do processes of one osteocyte establish contact with processes of the adjacent cells within the mineralized matrix?

Are the processes and canaliculi formed and contacts established after the osteocytes are embeded in the matrix or are they formed during the embedding process itself. Is there any evidence to suggest ...
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Size of classical liver lobule across species

A classical liver lobule is made up of a central vein and the portal triads. A typical human liver weighs around 3 lbs. , while a typical bovine liver weighs around 12 lbs. I was wondering if anyone ...
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168 views

What is the proper quick freezing /snap freezing protocol for pancreatic tissue?

I would like to do a quick freeze on pancreas from mice. I want to then make sections (30 ┬Ám thick). The idea is to preserve a fluorescent staining done in vivo. I do not know if I should place my ...
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Significance of basal lamina for outer layers of epithelium

In stratified (not pseudostratified) epithelia such as the epidermis, what purpose does the basal lamina serve for the outer layer cells which do not even stand in contact with it? Also, how do these ...
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What is the Giemsa staining of chromosomes?

I got the question in my exam and wrote the following and I do not understand what is wrong in it: Giemsa staining is a staining method to stain particularly malaria and other parasital diseases. ...
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What is the morphological difference between Leydig cell in human and pig?

The pig is only an example, just an animal. Leydig cells have protein inclusions (Reinke crystals) that are mostly made of crystallised lipofuscin. They are secretory inclusions i.e. cells formed in ...
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183 views

What histological stain can I use for beta-keratin?

I'm trying to find a histochemical stain for beta-keratin, the type found in Reptilia which is organized in beta-sheets. It's different than alpha-keratin which is found in mammalian skin, hair, ...