The tag has no usage guidance.

learn more… | top users | synonyms

17
votes
1answer
786 views

Can siRNA induce DNA methylation in mammalian cells?

Some years ago Hiroaki Kawasaki and Kazunari Taira published an article called "Induction of DNA methylation and gene silencing by short interfering RNAs in human cells" in Nature: In plants, ...
12
votes
1answer
606 views

How are epigenetic marks transmitted during cell division?

As far as I know, this is one of the biggest questions in the epigenetic field: how are the epigenetic marks like histone modifications propagated through cell division? A lot is already known about ...
4
votes
1answer
169 views

How does “inheritance of methylation” of DNA and/or histones work?

What are the current models/ideas describing the mechanisms explaining inheritance of methylation on DNA resp. histone level? Is there evidance of this "setup" information being really duplicated/...
3
votes
2answers
52 views

ChIP-seq for histone modification not in agreement with RNA-seq for expression

I have ChIP-seq for H3K79me2 and H3K36me3 and RNA-seq data for treated and untreated samples. Those two histones mark active genes. Lets say, hypothetically, a peak caller finds differential sites at ...
3
votes
1answer
34 views

Can one talk about deacetylation of a promoter rather than associated histone?

I am confused on a detail in a paper I am reading and am not sure whether I am misunderstanding the wording or misunderstanding the concept. I am including the whole abstract of this paper for ...
2
votes
1answer
33 views

H3K36me3 role in RNA polymerase II elongation

I found a paper describing the association of RNA polymerase II with the histone methyltransferase Set2 that methylates H3K36. At the end they say: While our results strongly implicate K36 ...
2
votes
1answer
37 views

What signals histone methyl transferases to function?

I wanted to find out if anyone knows what stimulates histone methyltransferases to function? I know that it is a process still trying to be understood, but I can't find anything that seems to direct ...
1
vote
2answers
78 views

How does mass spectrometry detect histone methylation?

In this research paper they talk about the methylation of histone. They use mass spectrometry to identify which peptide position is methylated. Does the number yielded by the spectrometer calculate ...
0
votes
0answers
18 views

Peak calling for TF and histone modification

I was viewing different methods for peak calling and came across two quite similar concepts but still different methods: MACS and SICER. The main difference is that SICER uses nonoverlapping sliding ...