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530 views

Gel electrophoresis after RNAse treatment

I do not understand how to solve this question. I know that RNAse will cut smaller pieces of RNA. The answer given is A
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1answer
212 views

recombination frequency problem

Three loci C, D and E are located on the same chromosome in this order. We found that the frequency of recombinants between C and D is 10% and that between D and E it is 20%. Assuming that ...
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2answers
110 views

Calcium levels and nerve hyperexcitation

Why does lower blood calcium levels (or lower calcium levels in ECF) cause nervous hyperexcitaton? Why does it cause over stimulation of nerves and muscles and spasmic contractions of muscles? This is ...
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1answer
70 views

Linkage distance calculation

What is the map unit distance between L and S allele ? My working : If we take the normal ratio :9:3:3:1 , first one should be 9/16 = 0.5625 and then subtract 0.51 from it, we get 0.0525, ...
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1answer
52 views

Identifying mutations in a yeast strain

Cells of a triple-mutant yeast strain (leu- his- trp-) were spread either on minimal medium or on minimal medium supplemented with various combinations of histidine, leucine, or tryptophan. The ...
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1answer
73 views

Blood group probability question

My answer ( after rounding off) is 9% -100( 0.75 X 0.25 X 0.5) but the answer given is 22 % Am I correct ?
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1answer
159 views

How can synonymous mutations lead to cancerous or tumorous phenotypes?

After analyzing DNA sequences of an oncogene from many human cancer patients, you found that synonymous substitutions occur in a specific codon of this oncogene. Assuming that these synonymous ...
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0answers
37 views

Paired-end mapping exercise

Pair-end mapping (PEM) is a technique that allows to detect structural variants in DNA by obtaining paired-end reads and the comparison of their positions in a reference genome. Then among libraries ...
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2answers
122 views

Which restriction enzyme would i use?

Plasmid pBr322 includes two genes that confer antibiotic resistance: a gene for ampicillin and a gene for tetracycline. The cutting site for the restriction enzyme BamH1 is in the middle pf the ...
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2answers
90 views

Identifying the inheritance pattern

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1answer
91 views

How HIV Affects and Its Treatment using Combination Therapy

Can someone please help me with the following questions. I've written my specific questions right after the text question. Human immunodeficiency virus (HIV) is a retrovirus. Its genome is a single ...
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4answers
265 views

Punnett Square Help

In fruit flies, red eyes are dominant over white eyes. Show a cross between two white-eye fruit flies. My question is... How do I know if the white-eye fruit flies are homozygous or heterozygous?
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1answer
76 views

Determining sequence of oligoribonucleotide

Oligoribonucleotide X was treated with phosphatase (for removal of 3' and 5' - terminal phosphates), then with RNAase T1, which cleaves all phosphodiester bonds located in a 3' position of ...
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1answer
21 views

Order of events in hibernation

Arrange this in sequence : i. Heat loss exceeds heat production. ii.As body temperature falls, heat loss decreases. iii.Body temperature equals environmental temperature. ...
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1answer
22 views

Is ability to easily pollinate desirable?

In Mendel's crosses was it desirable for the plants to be able to cross-pollinate and self pollinate easily ?
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1answer
345 views

Recombination frequency, linked genes, and independent assortment

Gene R is genetically linked to gene T with a recombination frequency of 0.3. Gene T and gene X assort independently from each other. Your colleague tells you that gene T and gene X reside on the same ...
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0answers
535 views

Interpreting graph of mitosis and meiosis

Note : Consider the y axis as 1,2,3,4(not 2,3,4) and x axis as time. Roman numerals should be considered from I-X ( some are not correct here ) Which stages show mitosis? My answer : III-V Which ...
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2answers
371 views

Gel electrophoresis question

Leena is a molecular biology student. She purifies two fragments of DNA, 800 and 300 base pairs long. These were obtained from a plasmid after digesting it with HindIII. Each of these fragments has a ...
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2answers
156 views

Hardy Weinberg Equilibrium problem

In a small tribal population, the frequencies of two alleles A and a at a particular locus were 0.3 and 0.7, respectively. However, not all the individuals with genotype aa could live up to the ...
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2answers
104 views

Protein purification methods

Which of the following methods would yield the most purified protein fraction? A. Salt precipitation B. Charge separation C. Affinity purification The first one is the most commonly used, is it ...
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2answers
142 views

Is AT or GC content important in electrophoresis?

Will it make a difference in running speed if we run samples of same no of bases but different AT - GC content ?
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3answers
8k views

Deciding between chi square and t test

I am always so confused whether to do a chi square test or a t test in the sums given by my biostats teacher. Does anyone have a simple rule to decide this?
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1answer
72 views

Electrophoretic mobility of isozymes

If isozymes are seperated using electrophoresis, which of the following will be the principle of separation ? A.charge density B.molecular weight C.polarity of molecule I think it should be B.
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1answer
30 views

Amphibian blood circulation

In an amphibian blood entering the right atrium shows higher levels of oxygenation than that in left atrium when : A.It is hyperactivated B.it is hibernating C.it is submerged D.it is in the ...
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3answers
63 views

Use of salt as preservative

I know that salt is used as preservative as it dehydrates microbes. Is there any other advantage like - altering pH or inactivating microbial enzymes ?
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1answer
182 views

Why can CO₂ be transported dissolved in plasma?

Is it because CO2 is more soluble in water or has it got to do with the alkaline pH of plasma ?I here mean only aqueous CO2 transport from body cells to lungs.
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2answers
275 views

Homework : Sodium Potassium pump

Why does sodium-potassium pump consume about 2/3 energy of a cell ? A.maintains appropriate membrane potential B.helps in co-transport I think it should be A.
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1answer
47 views

Proportion of amino acids from a random polymer of U and C

A protein synthesis assay was carried out in vitro. A polyribonucleotide containing U and C in proportion 1:5 (positions of U and C are random) was used as a template. Which amino acids and in ...
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2answers
142 views

Which statement about phenotypic plasticity is false?

This was a test question, and I'm not too sure about the answer. Which statement about phenotypic plasticity is false? a. Because the plasticity of a phenotypic trait may be costly to maintain, it ...
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0answers
44 views

Shortest route for a Nitrogen atom from fossil fuel to a protein in your body? [closed]

Millions of years ago fossil fuels originated from plants. A nitrogen atom from fossil fuel is now inside in a protein molecule in your body. What is the shortest path that the nitrogen atom ...
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1answer
125 views

Energy flow in an ecosystem

What are the mistakes in here? The image below shows a part of the energy flow diagram in an ecosystem. 1. Part P of the biomass of the primary producers does not get consumed. Give a concrete ...
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1answer
264 views

Genetic variation and sensitivity of wild cabbage

1: Is it true that the genetic variation of kale plants is smaller than wild cabbage? I think so, because of human selection of the kale plants. 2: Are broccoli and cauliflower more ...
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1answer
83 views

Which mountain range was formed first (historically) and which the pedigree is correct?

Source 1: Source 1 shows a map of a particular area. In this map you will find the mountain ranges 1 and 2, the species P, Q and R. Two possible pedigrees of the ancestral history of the species ...
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0answers
63 views

Why did the yeast rise?

Here are the notes I made for an experiment I was doing (I didn't put it here as it is too long) (please ignore the numbers at the end of the pdf file) The graph looks like this: As you see on ...
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1answer
62 views

Function of carapace

Majority of crustacean larvae have expanded carapace drawn out into processes as an adaptation for : a. Protection from enemies b. Planktonic habit The answer given is b but I think it should be ...
2
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1answer
248 views

Population Growth Rate Question?

A population of 600 birds faced a problem of biological magnification resulting in a large number of deaths reducing their population to 350. But 230 births also took place and 21 birds immigrated ...
0
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1answer
44 views

Reassociation of DNA

What is the order in which this DNA samples would would reassociate ? e.coli DNA plasmid DNA viral DNA satellite DNA DNA-RNA hybrid This is a homework question and I have not been given any ...
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1answer
111 views

cellular respiration

What are the effects of addition of these chemicals to actively respiring cells ? Which of them would lead to INHIBITION of aerobic respiration ? Would cytochromes be actually taken up by cells ? ...
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1answer
63 views

Shade-loving Plants

In shade-loving plants, the light compensation point is reached at low irradiance because: A. Leaves have more chlorophyll per reaction center B. These plants have greatly reduced respiration rates ...
4
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1answer
303 views

Hypotonic or Hypertonic solution and why?

A doctor needed to administer 5% albumin to a patient. Albumin is normally administered dissolved in 0.9% saline solution. Since the physician did not have any 5% albumin solution, he diluted 25% ...
0
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1answer
100 views

Lizards are sometimes called cold-blooded because they depend on their surrounding temperatures to keep them warm.

Predict how the composition of the cell membrane of a lizard might change from winter to summer. Justify predictions. Can someone please help me figure this out. Does it have to do with fluidity?
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1answer
56 views

Male behaviour during breeding season

The mountain blue bird build nests to attract females and exhibit aggression towards other males during breeding season. Which of the following is likely to give the birds maximal evolutionary ...
2
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1answer
32 views

Osmoregulatory Acclimatization in amphibians

If a frog is gradually exposed to increasing concentration of salts can they acclimatise or will they die of exosmosis ?
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1answer
120 views

Which is the correct statement on proteins?

I need help with one of the questions on my biochemistry assignment Choose the correct statement on proteins: a) proteins are folded by alpha-helix b) proteins can preserve their function even if ...
2
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2answers
173 views

Hardy Weinberg Equilibrium after 5 Generations

1 There is a population not at HWE where red eye = $a^+$ (dominant) and white eyes = $a^-$. if $a^+/a^+ = 0.6$ $a^-/a^+ = 0.1$ $a^-/a^- = 0.3$ what are the frequencies of the $a^+$ and $a^-$ ...
3
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1answer
286 views

Possible Genotypes of 4 Alleles of Adh

There are four alleles of Adh which are Adh-1, Adh-2, Adh-3 and Adh-4. Their respective frequencies are 0.11, 0.84, 0.01 and 0.04. What are the Hardy-Weinberg frequencies of the possible 10 ...
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2answers
50 views

3 fates of this matter

When an animal eats something, it obtains matter and energy right? But, what are the 3 possible fates of the matter and 3 possible fates energy. I don't understand what they are even asking. What ...
2
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1answer
165 views

Genetics problem-Height of a plant

An inbred strain of plants has a mean height of 24cm. A second strain of the same species also has a mean height of 24cm. When these plants are crossed the F1 are also 24cm in height.However, when the ...
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1answer
140 views

Why are centrioles aligned at 90 degree with each other?

The centrioles are aligned at 90 degree with each other. What is the function of this?
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0answers
43 views

What are the characteristic structures of bacillus M. tuberculosis and what they cause?

I answered to this question: In most forms of the disease, the bacillus M. tuberculosis spreads slowly and widely in the lungs, causing the formation of hard nodules (tubercles) in the ...