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How could a pure culture be represented by more than one ssu rRNA sequence?

Your lab works on an organism isolated from radioactive waste. However, you show that 3 very similar, but not quite identical, ssu-rRNA sequences can be amplified from the culture even though it is ...
2
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1answer
31 views

What range of dose should be used?

This is a dose-response experiment testing a new cancer drug. the darker line represents cancer cells. what range of dose should be used? I think it's 2-4 because this affects cancer cells only. is ...
2
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1answer
78 views

At what substrate concentration will an enzyme work at 40% of its maximum rate if its Km = 0.095M?

The question involves the Michaelis-Menten kinetics equation. Here is what I did: I took the Michaelis-Menten equation, replaced v with 0.4Vmax, canceled the Vmaxes (one on each side), and solved ...
2
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2answers
223 views

Translate DNA to Protein

Assuming the sequence shown is read left to right, what is the sequence of the protein produced? sequence: 5’-ATGTACTTCCATCTGGAATAG-3’ MY ATTEMPT: I know RNA is synthesized 5 to 3. This is ...
2
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1answer
43 views

Similarities Between Cells?

Which of the following are usually identical between a lung cell and a brain cell, from the same person, assuming that they are normal (non-cancerous) cells? Circle your choice(s). ...
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1answer
3k views

Which enzyme catalyzes transcription and which translation?

My attempt: Transcription - RNA polymerase Translation - aminoacyl tRNA synthetase I know that the ribosome almost runs translation, but I don't think ribosomes are enzymes, which is why I went ...
2
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1answer
71 views

Are there any organisms with larva more advanced than adult form?

Usually adult organisms are more sophisticated than corresponding larva, i. e. frogs, mosquitoes, fish. Are there species where the opposite is true?
2
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1answer
180 views

Are Bovine serum albumin, Avidin, Ficoll-70 and Dextran-70 positively charged or negatively charged?

Bovine serum albumin, Avidin, Ficoll-70 and Dextran-70,are they positively charged or negatively charged ? And which other solvents can be used as a substitute to water for preparing solutions in each ...
3
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1answer
797 views

Is secondary follicle or Graafian follicle arrested in the second metaphase of oogenesis?

My note about which I am not sure: Secondary oocyte is arrested in the metaphase II monthly after puberty until fertilization with sperm. The following picture also satisfies my sentence: . So ...
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1answer
121 views

How to manufacture different sized micelles in nano -scale?

I am trying to answer q5: "How can you manufacture micelles in A) nanometerer -scale B) and in ten nanometer -scale?" My Thinking Observations and some thinking Oil in ...
2
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1answer
219 views

What happens to colloidal particles in a liquid medium? And how to stabilize it?

Definition Colloid is a dispresion of small particles of one material in another. Definition 2 (Wikipedia) A colloidal system consists of two separate phases: a ...
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0answers
36 views

How many pandemic H1N1 2009 virus sequences were submitted by the Indian NIV as of May 10, 2010? [closed]

The handout I was following is maddening. It says; "Go to the NCBI home page and select 'All Resources (A-Z)' and choose GenBank. Follow the NCBI Flu Resource link to determine how many pandemic H1N1 ...
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2answers
2k views

Restriction Mapping of Plasmid Assignment

pUWL22 is a circular, double stranded, 10.5 kb plasmid. It contains a gene encoding an enzyme that confers ampicillin resistance in the host bacterium. Cloning into the kpn I and Sst I sites ...
3
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1answer
198 views

Predicting progeny of recessive mutations using recombination

I was asked this question on a test and got it wrong, but I'd like to know how to do it. The answers are shown in the blanks below: You are studying two recessive mutations in the fruit fly D. ...
4
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0answers
42 views

Determine which gene is in the middle complementation [duplicate]

Possible Duplicate: Crossing mutant strains on minimal media problem I'm studying this problem for my genetics final: My Attempt: The answer for part B is met2 and the answer for part C ...
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1answer
208 views

DNA chip contains a complete set of random hexanucleotide (6-mers) probes? (Solve) [closed]

can you tell me how to find solution to this question A DNA chip contains a complete set of random hexanucleotide (6-mers) probes. Out of the 4^6 = 4096 probes, how many will form perfect ...
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0answers
95 views

How to find Dmax (genetics problem) [closed]

My attempt: I'm not sure this is right but this is what i did. I found the allele frequencies to be: A=0.4 B-0.3 a=0.1 b=0.2 I think to find Dmax I just multiply the two alleles with the larges ...
8
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1answer
187 views

Finding DNA from Amino Acid sequence problem

My attempt: First I took the single letter AA codes and made them amino acids. So, the first one is Trp which is 5'-UGG-3'. From this I got the DNA sequence 3'-CCA-5'. However, the correct ...
4
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1answer
218 views

Finding exons in DNA problem

My attempt: I looked for the TACs because I thought this would be AUG in mRNA and ultimately Methionine (the start codon). But apparently, that's not how you do this problem. Im confused because ...
6
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2answers
604 views

What is the main general difference between Mitosis and Meiosis?

I found such a clause: The general principle is that mitosis creates somatic cells and meiosis creates germ cells. However, I cannot agree. Each gametogonium needs to go through mitosis before ...
2
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0answers
80 views

Mouse meta-globin mRNA problem

This is an mRNA strand of mouse meta-globin: 5'-ccccagauacggaauucgaau-3' A) Which small RNA (below) is most likely to regulate expression of meta-globin? ...
2
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1answer
127 views

Why are Birds and Reptiles with abundant yolk sac polyspermic?

I was given an explanation that birds and reptiles are polyspermic because they have an abundant yolk sac. But how does it explain the thing? Chicken as an adult is not using in my opinion yolk as an ...
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1answer
2k views

What are differences between formation of embryonic disc in chick and mammal embryo?

Exam question which got lowest average points in my university: 1/5 average. No markscheme available so my attempt below. I assume that embryonic disk prefers to germ disk such that Formation prefers ...
6
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1answer
191 views

Effect of histidine on the binding affinity of HisP

I was asked the following question by my teacher: A gene regulatory protein called HisP regulates the enzymes for histidine biosynthesis in the bacterium E. Coli. HisP is a protein whoes ...
4
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1answer
38 views

How do fibers control muscles?

My professor says Nervus troclearis IV somatomotor fibers supply musculus obliquus superior. and similarly for nervus oculomotorius III Nervus oculomotorius postganglionic fibers supply ...
3
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1answer
459 views

What are all possible vectors for unicellular human parasites?

I got the given question. They want the following pieces of information about protozoological human parasites: disease - parasite - vector. There are 10 proteogenic diseases at the Wikipedia. I ...
2
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1answer
350 views

Is Aspergillus clavatus an unicellular organism?

I could not find the piece of information form Burton Human Parasitology -book and not by Google. Yeast is divided to two phylums: one of them is Ascomycota. Aspergillus clavatus belong to the given ...
5
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1answer
486 views

Genetic carrier Pedigree of Recessive Traits

A human male and female couple with normal colored ears discover that, in both of their families, their fathers (who have normal ears) each had siblings with red ears. Red ears is a rare autosomal ...
3
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1answer
7k views

Pedigree Probability of Autosomal Recessive Trait

Here is a pedigree: The trait is autosomal recessive. The question is: What is the probability that the bottom 2 people (4 and 5) have a child with the trait? I tried doing ...
6
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1answer
457 views

What forms the human amniotic sac?

I am trying to understand the formation of embryonic disc for human and chick so the following question is related to here about formation of embryonic disc. I had thought that the amniotic sac forms ...
3
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1answer
252 views

Does chicken embryo form the disc called “discoblast” in the cleavage and blastulation of chicken?

This thread is related to my previous thread which is still unsolved mainly. I need to be able to compare chick and human cleavage and blastulation of zygote. My friend says that Chicken have ...
3
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1answer
250 views

Where does meiosis II of oogenesis end exactly in tuba uterina - uterus?

I know that metaphase II ends at ampulla tuba uterina, but I am not completely sure where the telophase II ends. Is it in the triangular section of cervix uterii? I just have an intuition that the ...
2
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1answer
262 views

What is the structure and function of chromosomes during interphase?

Ok, it seems to be easy but I have probably ignored something by accident. Interphase is the phase where things are growing and the preparation for cell division happens. Its stages G1, S and G2. DNA ...
4
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1answer
2k views

Do humans have Coelom?

Kimball Biology 5e says Coelom is the main body cavity of many animals. It is lined with an epithelium derived from mesoderm. Gilbert Embryonal Biology 9e says Coelom is the space between ...
5
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1answer
146 views

When does Coelom form exactly?

Related to my other question. I know that the coelom is derived from mesoderm. Coelom seems to form during organogenesis within 3rd and 8th week of embryogenesis. However, that answer is not either ...
4
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1answer
328 views

Does Amphibian embryo's blastocoel become a primitive yolk sac without yolk?

The mammalian blastocoel becomes yolk sac without yolk according to my professor. I have not found any evidence that such a thing happens in amphibians like frog. I need to be able to compare and ...
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1answer
171 views

Is this mammalian embryo blastocyst, gastrula or only phase between them?

The picture shows the formation of early blastocyst and late blastocyst. The middle embryo has embryoblast. My professor says that blastocyst in general has embryoblast and trophoblast. Embryoblast ...
2
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1answer
458 views

What does entering of sex chromosomes to gametes mean during meiosis I?

I am reading one answer about meiosis: During meiosis I, the sex chromosomes separate and enter different sperm or egg cells (gametes). I assume that sex chromosomes refer here to homologous ...
4
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1answer
271 views

What is the largest perennial herbaceous plant?

What is the largest perennial herbaceous plant? My guess would be some kind of banana or bamboo.
6
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1answer
306 views

To understand why satellite cells are genetically inactive in Barr body

DAPI is used as a stain for DNA heterochromatic and euchromatic regions. The Barr body is heterochromatic. In the slide of a normal human female cheek's somatic cells, there is apparently no other ...
2
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2answers
1k views

What is the Giemsa staining of chromosomes?

I got the question in my exam and wrote the following and I do not understand what is wrong in it: Giemsa staining is a staining method to stain particularly malaria and other parasital diseases. ...
2
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1answer
73 views

Substrates of cytochemical reactions in this immunostaining

Expression of extracellular protein Laminin 9 alpha-4 chain in human skeletal muscle. Indirect immunostaining with HRP immunostain marker. Ob.x40. I have unsuccessfully searched NCBI -database, JSTOR ...