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Substrates of cytochemical reactions in this immunostaining

Expression of extracellular protein Laminin 9 alpha-4 chain in human skeletal muscle. Indirect immunostaining with HRP immunostain marker. Ob.x40. I have unsuccessfully searched NCBI -database, JSTOR ...
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What is the optimal frame size for protein secondary structure prediction methods?

The whole question is What is the optimal frame size for the second and third generation protein secondary structure prediction methods? Justify your answer. I remember it has something to do ...