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Blebbistatin effect on vesicles

Blebbistatin is a drug that specifically inhibits the assembly of myosin in the cytoskeleton. What effect would you expect blebbistatin to have on intracellular vesicles? ...
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1answer
55 views

Which organisms have their cell wall made of polysaccharides and amino acids?

I came across a question which somewhat goes like In which of the following kingdom, most of the members have a cell wall made of polysaccharide and amino acids ? - Monera or Fungi. To me it ...
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1answer
22 views

Does mechanotransduction of cells play a role in the biocompatibility of titanium?

What exactly is mechanotransduction as the information varies between sources. Some sources indicate that mechanotransduction is the underlying principle where cells pull on the surface they grow on ...
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1answer
49 views

Why all trees have cylindrical stems?

Why a huge number of trees/plants have cylindrical stems? I have been told that this question's answer lies in Physics but I don't know where to find. Can any one help?
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1answer
71 views

Techniques to show a protein heterodimer-DNA interaction

It is known that c-Jun and fos dimerize to form AP1 factor that binds to a sequence on DNA containing PyPuGACGTCNNNNGAGGTCPyPU. In esophageal cancer cell lines there is no expression of the fos gene. ...
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0answers
33 views

Minichromosome maintenance protein structure and function

I am having difficulty answering three homework questions which relate directly to Chong et al. (2000). Questions The authors have determined that MtMCM is able to bind both ssDNA and dsDNA (see ...
3
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0answers
101 views

Cause-and-effect questions about growth and development of plant

I need to solve some cause-and-effect problems. The problems are related to growth and development of plant. "Growth and Development" chapter is the first chapter in third level of high school (senior ...
3
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0answers
51 views

Problem involving selfing (inbreeding it with itself) a plant to generate purebred lines

I am working on a past exam problem where the first bit is as follows A plant is repeatably selfed to generate inbred lines. Let $\mathbb{P}(He|He)$ denote the probability that a heterozygous ...
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0answers
39 views

Is there any mechanical digestion that occurs once food/chyme enters the small intestine?

Does mechanical digestion occur in small intestines and, if so, how does it occur? I found some sources that say digestion happens mechanically and chemically in the small bowel, and others that say ...
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0answers
34 views

How to gauge the clinical significance of specific cell type presence?

How does one decide whether the presence of certain cell types is clinically important or negligible? Would the presence of certain cells in conjunction with other symptoms be enough, or should it be ...
2
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0answers
235 views

How many proteins are synthesized in human body throughout life?

My idea to calculate this is: Define up to what age will do the calculation. (for instance 60) Determine how many cells born and died up to that point in life. (by type of cell) Determine how many ...
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0answers
41 views

Elevated transaminases after blood transfusion?

This sparks HCV immediately in my mind. However, there may be other possibilities too. What can you deduce from elevated transaminases if you only know that the healthy adult patient received blood ...
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0answers
18 views

Any books/publications about what life was like before antibiotics?

Just wondering. We've been discussing antibiotic resistant bacteria a bit in lecture, and how we're slowly sinking into a pre-antibiotic era again.
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0answers
122 views

Construct a restriction map of a linear fragment of DNA using the following data?

I've attempted to do the single digests, and the double digests, but cannot complete the map.... I've attached what I've done so far DNA Sizes of Fragments (bp) uncut DNA 900 DNA cut with EcoRI ...
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0answers
52 views

Which of the three is true for insulin receptors?

I have seen the following question in a Cell Biology exam: Which of the following is true" Insulin has an hydrophobic Signal Peptide and the insulin receptor does not have an hydrophobic ...
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0answers
39 views

Relation between heterozygosity and allelic diversity in founder effects/bottleneck?

Can someone try to explain me why allelic diversity falls faster than heterozygosity, reminding you that we're talking about bottleneck or a founder effect? Look at this graphic: It's clear to me ...
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0answers
11 views

Which fractions are enriched for siRNA cleavage activity by comparing electrophoresis?

Size exclusion column chromatography was used to separate the proteins in a Drosophila cell lysate to attempt to identify the protein complex responsible for processing long dsRNA into siRNAs. ...
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0answers
11 views

final control prior to transfecting an expression vector into a mammalian cell?

I have been asked a general question: Once I have cloned a full-length cDNA into an expression vector, what final important control must I do before I transfect this into an embryonic stem cell line? ...
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0answers
61 views

Which DNA fragments do not have expected sizes on this gel electrophoresis?

The problem is such: After performing a PCR, the vector carrying the PCR fragment with two restriction enzymes (Nhe1 and Asc1). The DNA samples were then separated using agrose gel electrophoresis ...
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0answers
27 views

height and bryophytes

Why does being taller provide advantages for Bryophyta but being shorter is better for Marchantiophyta? What disadvantages could being taller have? I think that by being taller, you have ...
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0answers
28 views

recombination between DNA segments question

In the diagram shown above, segments A and C are copies of a repeated DNA sequence, flanking a unique stretch shown as B. A and C are in an inverted orientation relative to each other, as indicated ...
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0answers
91 views

mutations induced by transposons

Question: In contrast to chemically-induced mutations, mutations induced by transposons are more likely to ... be lethal de dominant be stable revert to wild types be a gain of function The ...
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0answers
44 views

Chance of passing a risk allele to child?

Our genetics professor has posted up working for previous examination answers, but I am unsure that one of his answers is correct. My answer is close but may just be due to co-incidence. Question: ...
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0answers
71 views

Reasons for strange heart rate graph following exercise (line going up and down)?

Following a 2 minute general jumping and movement exercise designed to involve the entire body, the pulse was taken every minute for ten seconds. Strangely, the graph appears to jump up and down, for ...
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0answers
31 views

Invertase calculation?

If I wanted to make $500\mathrm{\mu g}/\mathrm{ml}$ of invertase, from Sigma's Invertase from Baker's yeast, which states is grade VII, and greater than or equal to $300 \mathrm{units}/\mathrm{ml}$, ...
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0answers
49 views

Calculating the recombination factor of four point genetic cross?

So, I'm trying to work out the recombination factor (RF) from this set of data: Which shows the 16 phenotypes of progeny of a cross of Saccharomyces cerevisiae yeast. I know how to go about a three ...
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0answers
140 views

Evolution of a Population

Scientists observe a newly established population of sexually reproducing plants growing on the shore of a small island. An observable trait of the plant has two possible phenotypes. It is determined ...
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0answers
96 views

How can we determine the polarity of fat molecules?

I'm trying to solve a question in a biochemistry quiz, which is asking for classifying a set of lipidic derivatives, by increasing polarity. Unfortunately, we didn't cover the classification of ...
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0answers
28 views

Genetic tests on S. cerevisiae to determine mutation locus on genes

I am studying the metabolism of galactose in the budding yeast Saccharomyces cerevisiae. After a random mutagenesis screen, several mutant strains were isolated that grow well in glucose but are ...
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0answers
31 views

Why do Sorghum Bicolor leaves roll up?

In A level Bio today we talked about abcesic acid as a stress hormone, and its ability to reduce osmotic potential around guard cells to close stomata. My question is, is abscesic acid in sorghum ...
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0answers
30 views

recombinant gametes

Suppose you are able to observe under a microscope the total number of meiotic divisions occurring in one gonad of a given individual and to outnumber exactly the crossovers between two given ...
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0answers
51 views

Paired-end mapping exercise

Pair-end mapping (PEM) is a technique that allows to detect structural variants in DNA by obtaining paired-end reads and the comparison of their positions in a reference genome. Then among libraries ...
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0answers
1k views

Interpreting graph of mitosis and meiosis

Note : Consider the y axis as 1,2,3,4(not 2,3,4) and x axis as time. Roman numerals should be considered from I-X ( some are not correct here ) Which stages show mitosis? My answer : III-V Which ...
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0answers
49 views

What are the characteristic structures of bacillus M. tuberculosis and what they cause?

I answered to this question: In most forms of the disease, the bacillus M. tuberculosis spreads slowly and widely in the lungs, causing the formation of hard nodules (tubercles) in the ...
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0answers
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What is the ramachandaran score of a denatured protein?

I thought it should be 0, but the professor pointed out I was wrong. What should the ramachandaran score of a denatured protein be?
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23 views

Rate of diffusion

I need to find out the intial diffusion rate across membrane. I know the formula of J=DA(C1-C2) but i am confused about how to make the calculations I also know the following: 1) Na+ concentrations ...
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0answers
15 views

Trouble identifying a gene given an Expressed sequence tag

I have an assignment where we need to answer several questions about an EST, however, I'm having trouble getting started. My sequence contained a Not I site (clipped that) and no poly A signal. We ...
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0answers
48 views

Rate of potassium/sodium transport

Assume that the plasma membrane of a cell was suddenly permeable to the same degree to both Na+ and K+ and that each responded to a concentration gradient of the same magnitude. Would you expect these ...
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0answers
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If spore possesses 10 chromosomes with 20 picogram DNA then calculate the amount of DNA in prophase II?

Options : a) 20 b) 10 c) 40 According to me, it should be 20 because a cell in prophase II is haploid after completing meiosis I reduction division. Also, spores are haploid, so amount of DNA should ...
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0answers
43 views

Animal Diversity Character State Matrix

i Need help wit developing a character matrix for all the kingdom animalia. i have spent many days trying to figure this out, but seem to get confused and lost.