0
votes
1answer
22 views

What attracts cells to: pathogens, professional antigen-presenting cells, and cells with an antigen on its MHC-1 protein?

I have a few questions in regards to attraction/stimulation in the immune system. What attracts leukocytes and antibodies to pathogens in the first place? What attracts CD4+ cells to professional ...
2
votes
1answer
18 views

Calibration curve for single radial immunodiffusion

When we draw a calibration curve for single radial immunodiffusion, the curve does not pass through origin. Instead, there is a y intercept. Why does that happen ? Shouldn't zero antigen give zero ...
2
votes
1answer
33 views

Why Rh conflict happen but no ABO conflicts?

I wonder why Rhesus conflict can happen during pregnancy and mother can make antibodies against Rh protein (I think the correct name is D protein), but it doesn't happen if mother has different ABO ...
3
votes
1answer
34 views

Dimerization of Immunoglobulin G

I would like to know the specific determinants for formation of IgG dimers. My understanding is the stem of the antibody is a homodimer of two heavy chains, covalently bonded through two disulfide ...
3
votes
1answer
151 views

What is the beneficial function of IgE antibody?

Dont tell me the "function" of IgE is to cause allergy ! In whatever texts I have seen it is written that IgE is important to cause allergies but what is the beneficial function of IgE ? Why was it ...
1
vote
1answer
43 views

Agglutination test using antibodies

Agglutination test Latex agglutination using bound antigens : by coating soluble (non - particulate ) antigens on to microscopic latex spheres, their reaction with a particular antibody can be ...
2
votes
2answers
216 views

Can more than one antibody bind the same antigen

I'd like to clear something up about antibodies that I'm not sure I've understood in the articles I've read. Looking at concepts such as "affinity maturation", "monovalent antigens" and "polyvalent ...
1
vote
2answers
38 views

Anti-antibodies for preventing transplant rejection

Transplanted organs from the donor are attacked by antibodies from the recepient. So can't we construct anti-antibodies and destroy specifically those antibodies which are causing the rejections ? ...
2
votes
0answers
15 views

Are there any antigen electronic sensors?

Is it possible somehow to attach antibody to silicon or to other electronic component chemical, and have electronic detector of correspondent antigen? Does such devices exist?
2
votes
1answer
338 views

Transfer of antibodies in breast milk of humans

Why isn't the IgA secreted in breast milk digested due to proteases of the digestive system in the baby? Wikipedia says: The secretory component of sIgA protects the immunoglobulin from being ...
1
vote
1answer
263 views

Transfer of antibodies from mother to fetus

My questions: When does fetus begins to acquire antibodies from mother? Does it continue throughout the pregnancy? From Wikipedia: http://en.wikipedia.org/wiki/Passive_immunity This occurs around ...
2
votes
1answer
54 views

Antibody production in secondary immune response

Can IgG antibodies be produced in the secondary immune response without the help of T cells? Is the affinity of antibody for antigens higher during secondary immune response?
5
votes
2answers
425 views

Possibility and feasibility of producing designed antibodies with bacteria

As stated here it is possible to produce fragments of antibodies in bacteria and harvest them (from the medium, I guess, but I don't have access to the full article). As it is possible to design ...
3
votes
2answers
149 views

How does the immune system distinguish own and foreign antibodies?

Therapeutic antibodies, for example Rituximab which recognises CD20 on B lymphoma cells, can cause adverse effects (e.g. http://www.ncbi.nlm.nih.gov/pubmed/19399690) One reason behind these adverse ...
3
votes
1answer
67 views

How to evaluate the efficacy of an antibody for fluorescence microscopy?

I'm trying to evaluate data taken from fluorescence microscopy with antibody staining, and am wondering whether there is any standard way to evaluate the specificity of the antibody for such ...
3
votes
2answers
189 views

variation in antigen binding site of antibodies

Antibodies or immunoglobulins are proteins made ​​by the immune system in response to alien(!) molecules. Each antibody binds to its specific antigen. This great diversity and specificity is cause of ...
8
votes
2answers
171 views

How are antibodies designed?

Antibodies have the ability of recognising highly specific peptide sequences and bind it at their antigen-binding site. This ability is harnessed as a tool in research to purify target structures in ...
8
votes
1answer
892 views

Is there a maximum amount of antibodies your body can keep?

I am wondering if you were theoretically able to get vaccinations or antibodies for any and every diseases and/or illness, would there be a limit to how many you can get and keep in your body at one ...