This tag is identical to enzyme-kinetics.

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Is it possible to measure Secondary Bile Acid Kinetics?

Is it possible to measure secondary bile acid kinetic and flux measurements in vivo? Anyone have any experience with this sort of technique?
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Incremental denaturation of protein mixtures

When a protein solution is heated above the denaturation temperature, it seems that denaturation does not happen at the time the temperature is reached, but it takes some time. I assumed that ...
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42 views

Change in synthesis rate of a molecule changes equilibrium concentration

I was reading the topic of 'The concentration of the molecule can be adjusted quickly only if the lifetime of a molecule is short' from Molecular Biology of the Cell by Alberts. At the end of pg-837 ...
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Prenatal hyperkinetic dysfunction and its effect on endurace

Do people with an hyperkinetic dysfuntion have a better endurance? Are they fitter in cause of their Dopamine-overdose or any other reason? I have a case, where someone does not do any sports, but ...
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62 views

Enzyme kinetics [closed]

I can't understand how to study enzyme kinetics. Say I have a lipase and want to study the kinetics of this lipase using a fluorogenic substrate, how would I do this? From what I understand I would ...
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phosphorous availability to mycorrhizae and parent plant, timing - paper search

I'm researching (for my work) phosphorous uptake in monocots to develop planting mixes for riparian planting. I recently saw a paper suggesting (the author lacked proof) that root zone mycorrhizae ...
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513 views

Why are enzyme-catalysed reactions slower at lower substrate concentration?

Suppose I'm using 200 nmoles of enzyme and 2 mmoles of substrate. The enzyme should be saturated but if I use 50 mmoles of substrate, the reaction will be faster. Why? I just can't get it! Even at ...
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1k views

Michaelis-Menten Kinetics: Does measuring apparent Km and Vmax take into account competing reactions?

I am learning about why it is important to measure Km and Vmax for each experimental setup because measuring the "apparent" Km and Vmax includes enzyme inhibitions of which one might not be aware. ...
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519 views

$S_{0.5}$ vs $K_m$ values in enzyme kinetics

What is the difference between $S_{0.5}$ values and $K_m$ values in enzyme kinetics?
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107 views

At what substrate concentration will an enzyme work at 40% of its maximum rate if its Km = 0.095M?

The question involves the Michaelis-Menten kinetics equation. Here is what I did: I took the Michaelis-Menten equation, replaced v with 0.4Vmax, canceled the Vmaxes (one on each side), and solved ...