Tagged Questions
5
votes
2answers
57 views
Is wiping with RNAse Zap enough to destroy RNAse activity?
From the RNAseZap MSDS, it is an SDS at some unknown concentration, maybe with some NaOH?
Some other links suggest there is some NaOH as well.
The Ambion site states that RNAseZap destroys RNAse ...
1
vote
0answers
32 views
Can I heat Trizol?
I wonder if I can heat Trizol reagent for 30 min 65C. The goal is to disrupt protein-RNA complex while inhibiting nucleases. (I can't use RNasin cause it's inactivated in 65C, and can't use RVC cause ...
5
votes
1answer
194 views
Agarose vs agar? Why do DNA gels use agarose only and how do you obtain agarose from agar?
Agar is a relatively cheap substance from red algae. And it contains a saccharide agarose as well as a small amount of pectin.
Agar is used for culture plates as is, but for DNA gels a grade of ...
3
votes
1answer
25 views
What is the extent of the effect of Tris on E. coli?
I was a fool and dissolved my antibiotic (Kanamycin) into Tris Buffer rather than H2O. The Kanamycin still seems to be active but a fellow labmate mentioned that Tris messes around with the membrane ...
