Chemicals used in the lab for experiments
7
votes
2answers
153 views
What are key factors when evaluating and comparing miniprep columns?
I'm looking to comparing different protocols for minipreps for plasmid DNA purification. What factors should I be looking at? A few things come to mind:
Cost
Yield
Time per step
Replacement with ...
5
votes
1answer
171 views
Agarose vs agar? Why do DNA gels use agarose only and how do you obtain agarose from agar?
Agar is a relatively cheap substance from red algae. And it contains a saccharide agarose as well as a small amount of pectin.
Agar is used for culture plates as is, but for DNA gels a grade of ...
5
votes
2answers
44 views
Is wiping with RNAse Zap enough to destroy RNAse activity?
From the RNAseZap MSDS, it is an SDS at some unknown concentration, maybe with some NaOH?
Some other links suggest there is some NaOH as well.
The Ambion site states that RNAseZap destroys RNAse ...
5
votes
1answer
83 views
What are good practices with reusing desalting columns
At least according to a few sources Prozyme and Protocols-Online, it is possible to reuse desalting columns and since I'm cheap I would like to also.
Key things seem to be washing with several column ...
4
votes
1answer
71 views
Is DNA green viewer carcinogenic?
I use DNA green viewer in Lab to see DNA and RNA bands. Some peers told me it is carcinogenic and is not safe.
Is this correct? If yes, are there better choices to use in working with gel?
3
votes
1answer
25 views
What is the extent of the effect of Tris on E. coli?
I was a fool and dissolved my antibiotic (Kanamycin) into Tris Buffer rather than H2O. The Kanamycin still seems to be active but a fellow labmate mentioned that Tris messes around with the membrane ...
1
vote
0answers
32 views
Can I heat Trizol?
I wonder if I can heat Trizol reagent for 30 min 65C. The goal is to disrupt protein-RNA complex while inhibiting nucleases. (I can't use RNasin cause it's inactivated in 65C, and can't use RVC cause ...
