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2
votes
1answer
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Strange behavior of a DNA gel
I ran a PCR product of ~300 bp on a 2% TAE-agarose gel for 30 minutes. I used Sybr-safe as a DNA stain. Voltage was 80V.
When I imaged the gel, the DNA on the bottom half of the gel, including the ...
8
votes
2answers
2k views
Why should I degas my gel solution for polyacrylamide gels?
In protocols for polyacrylamide gel electrophoresis (PAGE) I often see instructions to degas the gel solution by putting it under vacuum for 10-15 minutes before polymerizing the gel.
I usually ...
